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Baicalin production in transformed hairy root clones ofScutellaria baicalensis

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Abstract

A transformed hairy root clone ofScutellaria baicalensis was established following infection withAgrobacterium rhizogenes ATCC15834. Three root clones ofS baicalensis were selected by growth habit and baicalin content. The most active strain-the SR-03 clone-was examined for its growth and baicalin content under various culture conditions. The root growth and baicalin content were maximized in a Schenk and Hildebrandt medium supplemented with 4 and 6% sucrose, respectively. The accumulation of baicalin in transformed hairy roots was enhanced through exposure to various elicitors. Elicitation was attained by the addition of methyl jasmonate, salicylic acid, and various concentrations of fungal cell wasll elicitors to the medium. The accumulation of baicalin in the elicited cultures ranged from 10.5 to 18.3 mg/g dry weight of the roots, which was 1.5-to 3-fold the amount attained in controls.

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  1. Department of Oriental Medicine Materials, Dongshin University, 520-714, Naju, Korea

    Sung Jin Hwang

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Hwang, S.J. Baicalin production in transformed hairy root clones ofScutellaria baicalensis . Biotechnol. Bioprocess Eng. 11, 105–109 (2006). https://doi.org/10.1007/BF02931892

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