Abstract
Dielectric barrier discharge plasma was used to generate a stable strain of Klebsiella pneumoniae (designated to as Kp-M2) with improved 1,3-propanediol production. The specific activities of glycerol dehydrogenase, glycerol dehydatase and 1,3-propanediol oxidoreductase in the crude cell extract increased from 0.11, 9.2 and 0.15 U mg−1, respectively, for wild type to 0.67, 14.4 and 1.6 U mg−1 for Kp-M2. The glycerol flux of Kp-M2 was redistributed with the flux to the reductive pathway being increased by 20% in batch fermentation. The final 1,3-propanediol concentrations achieved by Kp-M2 in batch and fed-batch fermentations were 19.9 and 76.7 g l−1, respectively, which were higher than those of wild type (16.2 and 49.2 g l−1). The results suggested that dielectric barrier discharge plasma could be used as an effective approach to improve 1,3-propanediol production in K. pneumoniae.
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Acknowledgements
This work was supported by the National High Technology Research and Development Program of China (863 Program) (No. 2007AA02Z208) and the Major State Basic Research Development Program of China (973 Program) (No. 2007CB714306). The authors thank Mr. Tang Xiao-Hua for the screening of mutants, and Dr Alan K Chang for his help with the revision of the manuscript.
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Dong, XY., Xiu, ZL., Li, S. et al. Dielectric barrier discharge plasma as a novel approach for improving 1,3-propanediol production in Klebsiella pneumoniae . Biotechnol Lett 32, 1245–1250 (2010). https://doi.org/10.1007/s10529-010-0284-y
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DOI: https://doi.org/10.1007/s10529-010-0284-y