Abstract
A strain ofMicrococcus sp. was isolated for its capability of side chain degradation of cholesterol. This strain was characterized and identified asMicrococcus roseus. It was found to be the best strain for the production of androsta-1,4-diene-3, 17-dione and androst-4-ene-3, 17-dione compared with otherMicrococcus strains.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anonymous: Selective Isolation and Identification of Actinomycetes.Laboratory Manual—Protocol of Experiments Carried Out by the Participants of the Workshop Organised by IMTECH. IMT, Chandigarh (India) 1990.
Berger S.L., Kimmel A.R.: Guide to molecular cloning techniques.Meth. Enzymol.152, 165–166 (1987).
Bergey's Manual of Determinative Bacteriology, 8th ed. Williams & Wilkins, Baltimore 1974.
Brooks B.W., Murray R.G.E., Johnson J.L., Stackebrandt E., Woese C.R., Fox G.E.: Red pigmented micrococci: a basis for taxonomy.Internat. J. Syst. Bacteriol.30, 627–646 (1980).
Chattopadhyay M.K., Jagannadham M.V., Vairamani M., Shivaji S.B.: Carotenoid pigments of an Antarctic psychrotrophic bacteriumMicrococcus roseus, temperature dependent biosynthesis, structure, and interaction with synthetic membranes.Biochem. Biophys. Res. Commun.239, 85–90 (1997).
Conn H.J., Darrow M.A., Emmel V.M.:Staining Procedures Used by the Biological Stain Commission. University of Rochester Medical Center, Rochester, New York, 2nd ed., pp. 226–227. William and Wilkins. Baltimore 1960.
Cowan & Steel's Manual for the Identification of Medical Bacteria, 2nd ed. Cambridge University Press, Cambridge 1974.
Dogra N., Qazi G.N.: Biotransformation of cholesterol byMicrococcus species mediated by plasmid DNA.World J. Microbiol. Biotech.15, 411–415 (1999).
Harrigan W.F., McCance M.E.:Laboratory Methods in Food and Dairy Microbiology. Academic Press, London-New York-San Francisco 1966.
Mandel M., Marmur J.: Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA.Meth. Enzymol.12B, 195–206 (1968).
Martin C.K.A.: Sterols, pp. 79–95 in H.J. Rehm, G. Reed (Eds):Biotechnology, Vol. 6A. K. Kieslich-Verlag-Chemie, Weinheim (Germany)-Deexfield Beach (Florida, USA) 1984.
Microbial Identification System Operating Manual HP5898A. Hewlett-Packard, Newark, (Delaware, USA) 1992.
Miller J.H.:Experiments in Molecular Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1972.
Nagasawa M., Watanabe N., Hashiba H., Murakami M., Bae M., Tamura G., Arima K.: Microbial transformation of sterols. V. Inhibitors of microbial degradation of cholesterol.34, 838–844 (1970).
Vadalkar K., Gupta F.C., Bhat H.K., Chopra C.L.: Bioconversion of pregnenolone to 1,4-androstadiene-3,17-dione byArthrobacter simplex.Ind. J. Exp. Biol.18, 245–248 (1980).
Verma V., Qazi G.N., Prashad R., Chopra C.L.: An economical large scale procedure to purifyMicrococcus plasmid DNA.Biotechniques6, 936–940 (1988).
Wright M.A., Taylor F., Randles S.J., Brown D.E., Higgins I.J.: Biodegradation of a synthetic lubricant byMicrococcus roseus.Appl. Environ. Microbiol.59, 1072–1076 (1993).
Zohri A.A.: Progesterone transformation as a biochemical aid in classification of the genusEmericelle.Folia Microbiol.45, 391–396 (2000).
Zohri A.A., Abdel-Galil M.S.M.: Progesterone transformations by three species ofHumicola.Folia Microbiol.44, 277–282 (1999).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dogra, N., Qazi, G.N. Steroid biotransformation by different strains ofMicrococcus sp.. Folia Microbiol 46, 17–20 (2001). https://doi.org/10.1007/BF02825877
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02825877