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Secretory expression of K88 (F4) fimbrial adhesin FaeG by recombinant Lactococcus lactis for oral vaccination and its protective immune response in mice

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An Erratum to this article was published on 20 June 2009

Abstract

K88 (F4) fimbrial adhesin, FaeG, was expressed extracellularly in Lactococcus lactis using a nisin-controlled gene expression system. The antibody response and protective efficacy of the recombinant bacteria (L. lactis [spNZ8048-faeG]) against live enterotoxigenic E. coli (ETEC) C83549 challenge were evaluated in ICR mice. Mice vaccinated with L. lactis [spNZ8048-faeG] had a significantly increased antigen-specific IgG level in the serum and decreased mortality rate (P < 0.05) compared with the control. This indicates that oral immunization of L. lactis [spNZ8048-faeG] can induce an immune-response protection upon challenge with live ETEC in ICR mice.

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Acknowledgment

This study was supported by Zhejiang Provincial Science and Technology Foundation, China (No. 2006C12086).

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Authors and Affiliations

  1. Key Laboratory of Molecular Animal Nutrition of Ministry of Education, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, 310029, People’s Republic of China

    Chun Xia Hu, Zi Rong Xu, Wei Fen Li, Niu Dong & Ling Lin Fu

  2. Department of Life Science, Yuanpei College, Shaoxing University, Shaoxing, Zhejiang, 312000, People’s Republic of China

    Chun Xia Hu

  3. Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, 310029, People’s Republic of China

    Ping Lu

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  1. Chun Xia Hu
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  2. Zi Rong Xu
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  3. Wei Fen Li
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  4. Niu Dong
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  5. Ping Lu
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  6. Ling Lin Fu
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Correspondence to Wei Fen Li.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s10529-009-0045-y

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Hu, C.X., Xu, Z.R., Li, W.F. et al. Secretory expression of K88 (F4) fimbrial adhesin FaeG by recombinant Lactococcus lactis for oral vaccination and its protective immune response in mice. Biotechnol Lett 31, 991–997 (2009). https://doi.org/10.1007/s10529-009-9966-8

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  • DOI: https://doi.org/10.1007/s10529-009-9966-8

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