Abstract
A wheat β-1,3-glucanase gene (TaGluD) was identified as a fungal defense candidate. Its transcript induction was more than 60-fold higher in a resistant wheat line, Shannong0431, than in a susceptible wheat line, Wenmai6, after infection with Rhizoctonia cerealis. The TaGluD protein was overexpressed as inclusion bodies in Escherichia coli. After refolding and purification, TaGluD with 1 unit of β-1,3-glucanase showed antifungal activity in vitro against Rhizoctonia solani, R. cerealis, Phytophthora capsici and Alternaria longipes with inhibition rates of 42%, 43%, 32% and 30%, respectively. Thus TaGluD may be useful for enhancing fungal resistance in several crop species.
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Acknowledgments
This study was supported by the National “Hi-Tech” project (grant No 2006AA10A104) and the National “Key Scie-Tech” project (grant No. 2008ZX08002-001).
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Baoye Liu and Yan Lu have contributed equally to this work.
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Liu, B., Lu, Y., Xin, Z. et al. Identification and antifungal assay of a wheat β-1,3-glucanase. Biotechnol Lett 31, 1005–1010 (2009). https://doi.org/10.1007/s10529-009-9958-8
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DOI: https://doi.org/10.1007/s10529-009-9958-8