Abstract
Inulinase gene (Kcinu) derived from Kluyveromyces cicerisporus was expressed extracellularly in Kluyveromyces lactis using an episomal vector directed by Kcinu promoter. The influence of hap1 gene disruption on the expression of inulinase was studied. Inulinase activity in the supernatant of the recombinant Klhap1Δ strain was 391 U ml−1 after cultured 120 h, which was 2.2-fold that of the wild type host. The relative inulinase mRNA level of the Klhap1Δ strain was 11.3-fold that of the wild type strain, and the expression plasmid was more stable in the mutant host. Based on these results, the disruption of hap1 facilitated the high and stable expression of inulinase controlled by Kcinu promoter in K. lactis.
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Acknowledgments
This work was supported by grants from Chinese 863 Program (No. 2007AA02Z107) and Shanghai Major Basic Research Program (No. 08JC1401100) .
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Yu, J., Jiang, J., Fang, Z. et al. Enhanced expression of heterologous inulinase in Kluyveromyces lactis by disruption of hap1 gene. Biotechnol Lett 32, 507–512 (2010). https://doi.org/10.1007/s10529-009-0182-3
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DOI: https://doi.org/10.1007/s10529-009-0182-3