Abstract
Diastereoselectivity-enhanced mutants of l-threonine aldolase (l-TA) for l-threo-3,4-dihydroxyphenylserine (l-threo-DOPS) synthesis were isolated by error-prone PCR followed by a high-throughput screening. The most improved mutant was achieved from the mutant T3-3mm2, showing a 4-fold increase over the wild-type l-TA. When aldol condensation activity was examined using whole cells of T3-3mm2, its de was constantly maintained at 55% during the batch reactions for 80 h, yielding 3.8 mg l-threo-DOPS/ml.
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Acknowledgments
We thank Ms. Tomiki Aki for her technical assistance. This work was supported by partly Chonbuk National University.
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Gwon, HJ., Baik, SH. Diastereoselective synthesis of l-threo-3,4-dihydroxyphenylserine by low-specific l-threonine aldolase mutants. Biotechnol Lett 32, 143–149 (2010). https://doi.org/10.1007/s10529-009-0125-z
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DOI: https://doi.org/10.1007/s10529-009-0125-z