Abstract
A novel aqueous two-phase system consisted of 2-propanol/ammonium sulfate was used for the extraction of 2,3-butanediol from fermentation broths. The maximum partition coefficient and recovery of 2,3-butanediol reached 9.9 and 93.7%, respectively, and more than 99% of the cells and about 85% of the soluble proteins were removed when 34% (w/w) 2-propanol and 20% (w/w) ammonium sulfate were used. The separated cells could be re-used as inocula for subsequent fermentations. The aqueous two-phase system described in this study may have potential application in the extraction of 2,3-butanediol produced by industrial fermentation processes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Afschar AS, Vaz Rossell CE, Jonas R, Chanto AQ, Schaller K (1993) Microbial production and downstream processing of 2,3-butanediol. J Biotechnol 27:317–329
Antov MG, Peričin DM, Dašić MG (2006) Aqueous two-phase partitioning of xylanase produced by solid-state cultivation of Polyporus squamosus. Process Biochem 41:232–235
Dong JF, Lin JQ, Huang XD (2003) Solubility and liquid-liquid phase balance of isopropanol-ammonium sulfate-water system. J Jishou University 24:83–85 (in Chinese)
Garg SK, Jain A (1995) Fermentative production of 2,3-butanediol: a review. Bioresour Technol 51:103–109
Ghosh S, Swaminathan T (2004) Optimization of the phase system composition of aqueous two-phase system for extraction of 2,3-butanediol by theoretical formulation and using response surface methodology. Chem Biochem Eng Q 18(3):263–271
Hasmann FA, Santos VC, Gurpilhares DB, Pessoa-Junior A, Roberto L (2008) Aqueous two-phase extraction using thermoseparating copolymer: a new system for phenolic compounds removal from hemicellulosic hydrolysate. J Chem Technol Biotechnol 83:167–173
Ma BB, Zhang GL, Xu XL, Wang LW, Li C (2008) Fermentation of glycerol to 1,3-propanediol by domesticated Klebsiella pneumoniae XJPD-Li. Food Ferm Ind 34:37–39 (in Chinese)
Qin JY, Xiao ZJ, Ma CQ, Xie NZ, Liu PH, Xu P (2006) Production of 2,3-butanediol by Klebsiella pneumoniae using glucose and ammonium phosphate. Chin J Chem Eng 14(1):132–136
Syu MJ (2001) Biological production of 2,3-butanediol. Appl Microbiol Biotechnol 55:10–18
Wang H, Liu L, Guo YX, Dong YS, Zhang DJ, Xiu ZL (2007) Biotransformation of piceid in Polygonum cuspidatum to resveratrol by Aspergillus oryzae. Appl Microbiol Biotechnol 75:763–768
Wheat JA, Leslie JD, Tomkins RV, Mitton HE, Scott DS, Ledingham GA (1948) Production and properties of 2,3-butanediol. XXVIII. Pilot plant recovery of levo-2,3-butanediol from whole wheat mashes fermented by Aerobacillus polymyxa. Can J Res 26F:469–496
Xiu ZL, Zeng AP (2008) Present state and perspective of downstream processing of biologically produced 1,3-propanediol and 2,3-butanediol. Appl Microbiol Biotechnol 78:917–926
Zeng AP, Biebl H, Deckwer WD (1991) Production of 2,3-butanediol in a membrane bioreactor with cell recycle. Appl Microbiol Biotechnol 34:463–468
Acknowledgments
This work was supported by the grant from the Major State Basic Research Development Program of China (973 Program) (No.2007CB714306) and Teaching and Research Award Program for Outstanding Young Teachers in High Education Institutions of Ministry of Education of the People’s Republic of China. The authors thank Alan K Chang (Dalian University of Technology) for proof reading the manuscript.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Sun, LH., Jiang, B. & Xiu, ZL. Aqueous two-phase extraction of 2,3-butanediol from fermentation broths by isopropanol/ammonium sulfate system. Biotechnol Lett 31, 371–376 (2009). https://doi.org/10.1007/s10529-008-9874-3
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-008-9874-3