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Complementary analysis of microRNA and mRNA expression during phorbol 12-myristate 13-acetate (TPA)-induced differentiation of HL-60 cells

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Abstract

MicroRNAs (miRNAs) and mRNAs constitute an important part of gene regulatory networks, influencing diverse biological phenomena. To discover novel regulatory pathways during myeloid differentiation, we performed miRNA as well as mRNA expression profiling of in vitro-differentiating HL-60 cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA). The main findings were up-regulation of miR-146a/b, miR-21, miR-221, miR-222, miR-155, miR-26a and down-regulation of miR-199a*, miR-181c, miR-142-3p, miR-92. After integrating the miRNA and mRNA expression data into a Transcriptome Interaction Database by Molecule Annotation System (MAS) software, a number of differently expressed mRNAs were revealed as potential targets of these miRNAs.

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Acknowledgements

This work was supported by a National Hi-Tech Program of China Grant (No. 2006AA020701).

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Authors and Affiliations

  1. Medical Systems Biology Research Center, Tsinghua University School of Medicine, Beijing, 100084, Peoples’ Republic of China

    Ailiang Chen, Jian Yu, Tuo Deng, Yuxiang Zhou, Keith Mitchelson & Jing Cheng

  2. Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084, Peoples’ Republic of China

    Ailiang Chen, Jian Yu, Tuo Deng, Yuxiang Zhou & Jing Cheng

  3. National Engineering Research Center for Beijing Biochip Technology & CapitalBio Corporation, 18 Life Science Parkway, Changping District, Beijing, 102206, Peoples’ Republic of China

    Ailiang Chen, Mingyong Luo, Jian Yu, Tuo Deng, Liang Zhang, Yuxiang Zhou, Keith Mitchelson & Jing Cheng

  4. Institute of Rheumatology and Immunology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, 637000, Peoples’ Republic of China

    Guohua Yuan

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  1. Ailiang Chen
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  2. Mingyong Luo
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  3. Guohua Yuan
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  4. Jian Yu
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  5. Tuo Deng
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  6. Liang Zhang
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  7. Yuxiang Zhou
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  8. Keith Mitchelson
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  9. Jing Cheng
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Correspondence to Keith Mitchelson or Jing Cheng.

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Chen, A., Luo, M., Yuan, G. et al. Complementary analysis of microRNA and mRNA expression during phorbol 12-myristate 13-acetate (TPA)-induced differentiation of HL-60 cells. Biotechnol Lett 30, 2045–2052 (2008). https://doi.org/10.1007/s10529-008-9800-8

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  • DOI: https://doi.org/10.1007/s10529-008-9800-8

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