Abstract
Hydroperoxide lyase activity was found in sugar beet leaves. Its optimum pH and temperature were, respectively, 6.7 and 22°C. Under these conditions, conversion of linolenic acid 13-hydroperoxide to cis-3-hexenal with a maximum yield of 80% was reached after only 2 min. The stability of cis-3-hexenal was improved by acidifying the reaction medium. Based on these studies, a bioprocess producing green-note aldehydes in a laboratory-scale was achieved.
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Acknowledgement
This work is financed by the Agricultural General Direction—Walloon Region of Belgium. Ongena M is research associate at the FRS-FNRS in Belgium. Gigot C is recipient of a FRIA grant from the FRS-FNRS.
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The authors Holy N. Rabetafika and Cédric Gigot contributed equally.
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Rabetafika, H.N., Gigot, C., Fauconnier, ML. et al. Sugar beet leaves as new source of hydroperoxide lyase in a bioprocess producing green-note aldehydes. Biotechnol Lett 30, 1115–1119 (2008). https://doi.org/10.1007/s10529-008-9652-2
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DOI: https://doi.org/10.1007/s10529-008-9652-2