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Growth phase-dependent changes in the expression of global regulatory genes and associated metabolic pathways in Escherichia coli

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Abstract

Metabolic regulation in Escherichia coli was studied in terms of the changes in the expression of the global regulatory genes rpoD, rpoS, soxRS, cra, fadR, iclR and arcA at three different growth phases, in batch culture. The expression of rpoS and several rpoS-dependent metabolic pathway genes, such as tktB, talA, fumC, acnA, sucA, acs and sodC, were increased (∼1.5 to 2-fold) as the cells entered the late phase of growth. The changes in the expression of other global regulators and their effects on different metabolic pathway genes were less significant, as compared to rpoS, during the later phases of growth.

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Acknowledgement

We would like to thank Prof. Mori of Keio University, Japan for providing us with the E. coli strain BW25113. Thanks are also due to Brie Fuqua of UC Berkeley, CA, USA for editing the manuscript.

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Authors and Affiliations

  1. Department of Bioscience and Bioinformatics, Faculty of Computer Science and System Engineering, Kyushu Institute of Technology, Iizuka, Fukuoka, 820-8502, Japan

    Mahbuba Rahman, Mohammad Rubayet Hasan & Kazuyuki Shimizu

  2. Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan

    Kazuyuki Shimizu

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  1. Mahbuba Rahman
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  2. Mohammad Rubayet Hasan
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  3. Kazuyuki Shimizu
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Correspondence to Kazuyuki Shimizu.

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Rahman, M., Hasan, M.R. & Shimizu, K. Growth phase-dependent changes in the expression of global regulatory genes and associated metabolic pathways in Escherichia coli . Biotechnol Lett 30, 853–860 (2008). https://doi.org/10.1007/s10529-007-9621-1

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  • DOI: https://doi.org/10.1007/s10529-007-9621-1

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