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Construction of a bacterial artificial chromosome (BAC) library of common wild rice (Oryza rufipogon Griff.) for map-based cloning of genes selected during the domestication of rice

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Abstract

As a prerequisite for the map-based cloning of genes from common wild rice (Oryza rufipogon Griff.), which plays an important role in the domestication of cultivated rice (O. sativa L.), we constructed a median-insert size bacterial artificial chromosome (BAC) library of the common wild rice isolate, YJCWR, collected from Yuanjiang, Yunnan Province, China. The library consists of 52,992 clones, with an average insert size of 50 kb, and all clones were pooled into 46 three-dimensional super-pools to facilitate library screening through the PCR method. Seventeen candidate clones were isolated by five markers and some clones containing putative target regions were sequenced. Furthermore, in analyzing the sequences of YJCWR, a retrotransposon, SZ-55, that might contribute to the evolution of Oryza was found.

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Acknowledgments

The authors would like to thank Yongqiao Sun, Dandan He, and Zhengpeng Zhang, of the Beijing Genome Institution (BGI), for their helpful suggestions about BAC library construction and pooling. We would also like to thank Dr. Xiaowei Zhang and Dr. Bin Zhang of BGI for their assistance in BAC sequencing and assembly. This work was funded by a grant from the Conservation and Utilization of Agricultural Wild Plants Program of the Ministry of Agriculture of China, and by a grant from the China National High-Tech Research and Development (“863”) Program.

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Authors and Affiliations

  1. Department of Plant Genetics and Breeding and State Key Laboratory of Plant Physiology and Biochemistry, China Agricultural University, Beijing, 100094, China

    Xianran Li, LuBin Tan, Zuofeng Zhu & Chuanqing Sun

  2. Laboratory of Crop Heterosis and Utilization, Ministry of Education, Beijing, 100094, China

    Xianran Li, LuBin Tan, Zuofeng Zhu & Chuanqing Sun

  3. Beijing Key Laboratory of Crop Genetic Improvement, Beijing, 100094, China

    Xianran Li, LuBin Tan, Zuofeng Zhu & Chuanqing Sun

  4. Key Laboratory of Crop Genetic Improvement and Genome, Ministry of Agriculture, Beijing, 100094, China

    Xianran Li, LuBin Tan, Zuofeng Zhu & Chuanqing Sun

  5. Beijing Genomics Institute, Chinese Academy of Sciences, Beijing, 101300, China

    Haiyan Huang, Chenji Li & Songnian Hu

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  1. Xianran Li
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  2. LuBin Tan
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  3. Haiyan Huang
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  4. Zuofeng Zhu
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  5. Chenji Li
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  6. Songnian Hu
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  7. Chuanqing Sun
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Correspondence to Chuanqing Sun.

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Li, X., Tan, L., Huang, H. et al. Construction of a bacterial artificial chromosome (BAC) library of common wild rice (Oryza rufipogon Griff.) for map-based cloning of genes selected during the domestication of rice. Biotechnol Lett 30, 555–561 (2008). https://doi.org/10.1007/s10529-007-9558-4

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  • DOI: https://doi.org/10.1007/s10529-007-9558-4

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