Abstract
The transfer of the IncP-7 carbazole degradative plasmid pCAR1 from Pseudomonas putida SM1443 (derived from strain KT2440) into bacteria of river water samples was monitored using a reporter gene encoding red fluorescent protein (RFP). The number of transconjugants drastically increased in the presence of carbazole, and most appeared to belong to the genus Pseudomonas. The results suggest that the presence of carbazole benefits the appearance of transconjugants belonging to the genus Pseudomonas. Intriguingly, we also detected the transfer of pCAR1 into non-Pseudomonas, Stenotrophomonas-like bacteria.
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Acknowledgements
We are most grateful to Professor Dr. Molin Sølen of the Technical University of Denmark and associate professor Dr. Eva Top of the University of Idaho for providing pSM1833 and P. putida SM1443. This study was supported by the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN) in Japan and a Grant-in-Aid (Hazardous Chemicals) from the Ministry of Agriculture, Forestry, and Fisheries of Japan (HC-07-2325-2).
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Shintani, M., Fukushima, N., Tezuka, M. et al. Conjugative transfer of the IncP-7 carbazole degradative plasmid, pCAR1, in river water samples. Biotechnol Lett 30, 117–122 (2008). https://doi.org/10.1007/s10529-007-9519-y
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DOI: https://doi.org/10.1007/s10529-007-9519-y