Abstract
A binary vector containing two reporter gene cassettes has been developed. This vector is ideal for optimising new plant transformation systems. Following optimisation, one of the reporter genes can be replaced with a gene of interest; the second can be used as a marker to confirm transgenic lines, and to estimate locus number and determine zygosity. This allows simple, efficient and economical screening for homozygous single-insert lines and azygous controls.
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Acknowledgements
This research was supported by Biotechnology and Biological Sciences Research Council grant BBS/B/12172 (CEV and PMM), ARC Centre of Excellence grant CEO348212 (PMG and CEV), the University of Queensland Strategic Research Fund and the Queensland Government Smart State Initiative (PMG) and the Cooperative Research Centre for Tropical Plant Protection (PMS).
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Vickers, C.E., Schenk, P.M., Li, D. et al. pGFPGUSPlus, a new binary vector for gene expression studies and optimising transformation systems in plants. Biotechnol Lett 29, 1793–1796 (2007). https://doi.org/10.1007/s10529-007-9467-6
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DOI: https://doi.org/10.1007/s10529-007-9467-6