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Oral administration of a fusion protein containing eight GLP-1 analogues produced in Escherichia coli BL21(DE3) in streptozotocin-induced diabetic rats

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Abstract

Glucagon-like peptide-1 (7–36) amide (GLP-1), a gut hormone released into the blood stream after feeding, can stimulate insulin secretion by potentiating the insulinotropic action of glucose. An expression vector pET-22bG8, encoding a fusion protein containing eight tandem repeat GLP-1 ([Ser8, Gln26, Asp34]-GLP-1) analogues, was constructed and transformed into the Escherichia coli BL21(DE3) strain over-expressing the His-tagged fusion protein under the IPTG promoter. SDS-PAGE and Western blot analysis demonstrated that the His-tagged GLP-1 fusion protein migrated as a single protein with a molecular weight of 32 kDa. Following chronic (10 days) oral administration (20 mg kg−1 day−1) of the fusion protein to diabetic rats, serum glucose levels were significantly lowered from 26 ± 2.5 to 7.9 ± 1.4 mmol/l. Further studies are needed to evaluate the potential use for GLP-1 analogue short peptide in the treatment of diabetes mellitus.

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Acknowledgement

This research was supported by 863 Project funds (No. 2002AA213061) in P. R. China.

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Authors and Affiliations

  1. The Key Laboratory for Bioactivematerial of Ministry of Education, Institute for Molecular Biology, College of Life Science, Nankai University, Tianjin, 300071, P.R. China

    Jianhua Hou, Ruixiang Yan, Dongfeng Ding, Liquan Yang, Cuiyan Wang, Zhiqiang Wu, Xiaoju Yu, Weidong Li & Minggang Li

  2. Tianjin State Laboratory of Microbial Functional Genomics, TEDA College, Nankai University, Tianjin, 300071, P.R. China

    Jianhua Hou, Ruixiang Yan, Dongfeng Ding, Liquan Yang, Cuiyan Wang, Zhiqiang Wu, Xiaoju Yu, Weidong Li & Minggang Li

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  1. Jianhua Hou
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  2. Ruixiang Yan
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  3. Dongfeng Ding
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  4. Liquan Yang
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  5. Cuiyan Wang
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  6. Zhiqiang Wu
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  7. Xiaoju Yu
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  8. Weidong Li
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  9. Minggang Li
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Corresponding author

Correspondence to Minggang Li.

Additional information

J. H. Hou and R. X. Yan contributed equally to this work.

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Hou, J., Yan, R., Ding, D. et al. Oral administration of a fusion protein containing eight GLP-1 analogues produced in Escherichia coli BL21(DE3) in streptozotocin-induced diabetic rats. Biotechnol Lett 29, 1439–1446 (2007). https://doi.org/10.1007/s10529-007-9427-1

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  • DOI: https://doi.org/10.1007/s10529-007-9427-1

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