Abstract
A supramolecular approach was used for adsorbing a monolayer of adamantane-modified phenylalanine dehydrogenase on β-cyclodextrin-coated Au electrodes. The enzyme electrode (poised at +200 mV vs. Ag/AgCl) showed a linear amperometric response up to 3 mM l-phenylalanine (l-Phe) with a lower detection limit of 15 μM. The reversible nature of this immobilization approach was confirmed.
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Acknowledgments
This research was supported by grants from The Japan Society for the Promotion of Sciences to R. Villalonga and Y. Asano (Grant S-04257), and from Toyama Medical-Bio Cluster (The Ministry of Education, Culture, Sports, Science and Technology, Japan) to Y. Asano and S. Tachibana. Financial support to R. Villalonga from the International Foundation for Science, Stockholm, Sweden, and the Organisation for the Prohibition of Chemical Weapons, The Hague, The Netherlands (Grant F/3004-2) is also acknowledged.
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Villalonga, R., Fujii, A., Shinohara, H. et al. Supramolecular-mediated immobilization of l-phenylalanine dehydrogenase on cyclodextrin-coated Au electrodes for biosensor applications. Biotechnol Lett 29, 447–452 (2007). https://doi.org/10.1007/s10529-006-9259-4
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DOI: https://doi.org/10.1007/s10529-006-9259-4