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Expression of a carbonic anhydrase gene is induced by environmental stresses in Rice (Oryza sativa L.)

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Abstract

Expression of the gene (OsCA1) coding for carbonic anhydrase (CA) in leaves and roots of rice was induced by environmental stresses from salts (NaCl, NaHCO3 and Na2CO3), and osmotic stress (10%, w/v, PEG 6000). CA activity of rice seedlings more than doubled under some of these stresses. Transgenic Arabidopsis over-expressing OsCA1 had a greater salt tolerance at the seedling stage than wild-type plants in 1/2 MS medium with 5 mM NaHCO3, 50 mM NaCl, on 100 mM NaCl. Thus CA expression responds to environmental stresses and is related to stress tolerance in rice.

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References

  • Atkins CA, Patterson BD, Graham D (1972) Plant carbonic anhydrase. I. Distribution of type among species. Plant Physiol 50:214–217

    CAS  PubMed  Google Scholar 

  • Bagder MR, Price GD (1994). The role of carbonic anhydrase in photosynthesis. Annu Rev Plant Physiol Plant Mol Biol 45:369–392

    Article  Google Scholar 

  • Bageshwar UK, Premkumar L, Gokhman I, Savchenko T, Sussman J, Zamir A (2004) Natural protein engineering: a uniquely salt-tolerant, but not halophilic, α-type carbonic anhydrase from algae proliferating in low-to hyper-saline environments. Protein Eng Des Sel 17:191–200

    Article  CAS  PubMed  Google Scholar 

  • Bechtold N, Pelletier G (1998) In plants Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration. Methods Mol Biol 82:259–266

    CAS  PubMed  Google Scholar 

  • Hatch MD, Burnell JN (1990) Carbonic anhydrase activity in leaves and its role in the first step of C4 photosynthesis. Plant Physiol 93:825–828

    Article  CAS  PubMed  Google Scholar 

  • Hewett-Emmett D, Tashian RE (1996) Functional diversity, conservation and convergence in the evolution of the α-, β-, and γ-carbonic anhydrase gene families. Mol Phylogenet Evol 5:50–77

    Article  CAS  PubMed  Google Scholar 

  • Karlsson J, Clarke AK, Chen ZY, Hugghins SY, Park YI, Husic HD, Moroney JV, Samuelsson G (1998) A novel α-type carbonic anhydrase associated with the thylakoid membrane in Chlamydomonas reinhardtii is required for growth at ambient CO2. EMBO J 17:1208–1216

    Article  CAS  PubMed  Google Scholar 

  • Moroney JV, Bartlett SG, Samuelsson G (2001) Carbonic anhydrases in plants and algae. Plant Cell Environ 24:141–153

    Article  CAS  Google Scholar 

  • Moskvin OV, Ivanov BN, Ignatova LK, Kollmeier MA (2000) Light-induced stimulation of carbonic anhydrase activity in pea thylakoids. FEBS Lett 470:375–377

    Article  CAS  PubMed  Google Scholar 

  • Price GD, von Caemmerer S, Evans JR, Yu JW, Lloyd J, Oja V, Kell P, Harrison K, Gallagher A, Badger MR (1994) Specific reduction of chloroplast carbonic anhydrase activity by antisense RNA in transgenic tobacco plants has a minor effect on photosynthesis CO2 assimilation. Planta 193:331–340

    Article  CAS  Google Scholar 

  • Roberts SB, Lane TW, Morel FMM (1997) Carbonic anhydrase in the marine diatom Thalassiosira weissflogii (Bacillariophyceae). J Phycol 33:845–850

    Article  CAS  Google Scholar 

  • Sasaki H, Hirose T, Watanabe Y, Ohsugi R (1998) Carbonic anhydrase activity and CO2 transfer resistance in Zn-deficient rice leaves. Plant Physiol 118:929–934

    Article  CAS  PubMed  Google Scholar 

  • Smith KS, Ferry JG (2000) Prokaryotic carbonic anhydrases. FEMS Microbiol Rev 24:335–366

    Article  CAS  PubMed  Google Scholar 

  • So AK, Espie GS, Williams EB, Shively JM, Heinhorst S, Cannon GC (2004) A novel evolutionary lineage of carbonic anhydrase (ε-class) is a component of the carboxysome shell. J Bacteriol 186:623–630

    Article  CAS  PubMed  Google Scholar 

  • Tashian RE (1989) The carbonic anhydrase: widening perspectives on their evolution, expression and function. Bioessays 10:186–192

    Article  CAS  PubMed  Google Scholar 

  • Wang ZQ, Zhu SQ, Yu RP, Li LQ, Shen GR (1993) The saline-alkali of China. The Science Press 1–305

  • Wilbur KM, Anderson NG (1948) Electrometric and colorimetric determination of carbonic anhydrase. J Biol Chem 176:147–154

    CAS  PubMed  Google Scholar 

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Acknowledgments

This work was supported by the National High Technology Research and Development Program (863 Program) from the People’s Republic of China, and the Excellent Young Teachers Program in Higher Education Institute MOE, P. R. C.

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Authors and Affiliations

  1. Alkali Soil Natural Environmental Science Center (ASNESC), Stress Molecular Biology Laboratory, Northeast Forestry University, Harbin, 150040, P.R. China

    Song Yu, Xinxin Zhang, Qingjie Guan & Shenkui Liu

  2. Asian Natural Environment Science Center (ANESC), The University of Tokyo, 1-1-1 Midori-Cho, Nishitokyo, Tokyo, 188-0002, Japan

    Xinxin Zhang & Tetsuo Takano

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  1. Song Yu
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  2. Xinxin Zhang
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  3. Qingjie Guan
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  5. Shenkui Liu
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Correspondence to Shenkui Liu.

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Yu, S., Zhang, X., Guan, Q. et al. Expression of a carbonic anhydrase gene is induced by environmental stresses in Rice (Oryza sativa L.). Biotechnol Lett 29, 89–94 (2007). https://doi.org/10.1007/s10529-006-9199-z

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  • DOI: https://doi.org/10.1007/s10529-006-9199-z

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