Abstract
In this paper, sodium alginate-sodium carboxymethyl cellulose (SA-CMC) composite material was used as a carrier, and sodium alginate-embedded laccase (Lac@SC) was prepared by traditional embedding method. After that, ethylene glycol diglycidyl ether (EGDE) and glutaraldehyde (GLU) were used as cross-linking agents, two different cross-linking-embedded co-immobilized laccases (Lac@SCG and Lac@SCE) were innovatively prepared, respectively, and then these immobilized laccases were characterized by SEM, FT-IR and XRD, and the stability of the three immobilized laccases was explored. In addition, the effects of different factors on the removal of 2,4-DCP by immobilized laccase were studied, and the degradation kinetic models of three immobilized laccases on 2,4-DCP were summarized, the possible degradation pathways of pollutants were also given. Experimental results showed that compared to free laccase, the pH stability, thermal stability and storage stability of immobilized laccase were greatly improved. These immobilized laccases could maintain high activity at pH3~6, 45~55 °C. Lac@SCG had the best storage stability. After 30 days of storage, the relative enzyme activity was still more than 40%. Lac@SC had good reusability, the relative enzyme activity was still more than 50% after 5 uses. In the degradation of 2,4-DCP, all three immobilized laccases showed good performance, when Lac@SCE was at pH5, 35 °C, 25 h, the removal rate of 2,4-DCP could reach 95.2%; When at 45 °C, Lac@SC had the highest degradation rate which reach to 94%; At 45 °C, the degradation rate of Lac@SCG reached 83.2%.
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Acknowledgements
This work was supported by Science and Technology Innovation Team Project of Hubei Provincial Department of Education (Grant No. T2020002), Wuhan Science and Technology Planning Project (Grant No. 2020020601012274), National Natural Science Foundation of China (Grant No. 41571306) and Hubei Technological Innovation Special Fund (Grant No. 2020ZYYD019).
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Zhao, Z., Ren, D., Zhuang, M. et al. Degradation of 2,4-DCP by the immobilized laccase on the carrier of sodium alginate-sodium carboxymethyl cellulose. Bioprocess Biosyst Eng 45, 1739–1751 (2022). https://doi.org/10.1007/s00449-022-02783-z
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DOI: https://doi.org/10.1007/s00449-022-02783-z