Abstract
The most economically significant Chinese cotton cultivar (Gossypium hirsutum L. cv. Zhongmian 35) was transformed via Agrobacterium tumefaciens-mediated DNA transfer. The aroA-M1 gene that confers resistance to the glyphosate was fused with a chloroplast-transit peptide of Arabidopsis thaliana 5-enolpyruvyl-3-phosphoshikimate synthase (ASP) and expressed in cotton plants under the control of a CaMV35S promoter. Transgenic plants were directly selected on medium containing glyphosate. Thirty-four independent transgenic lines were obtained after selection, giving a maximal 1.9% transformation frequency. The integration and expression of the aroA-M1 gene in T0 plants and T1 progeny were confirmed using DNA hybridization, Western blot and PCR techniques. An increased resistance of T0 and T1 transgenic plants towards glyphosate was also observed.
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This research was supported by National Natural Science Foundation of China 30470913 and Natural Science Foundation of Guangdong Province 04105513.
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Zhao, FY., Li, YF. & Xu, P. Agrobacterium-mediated transformation of cotton (Gossypium hirsutum L. cv. Zhongmian 35) using glyphosate as a selectable marker. Biotechnol Lett 28, 1199–1207 (2006). https://doi.org/10.1007/s10529-006-9078-7
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DOI: https://doi.org/10.1007/s10529-006-9078-7