Abstract
To improve biogas yield and methane content in anaerobic digestion of excess sludge from the wastewater treatment plant, the sludge was disintegrated by using various methods (sonication, alkaline and thermal treatments). Since disintegrated sludge contains a high concentration of soluble proteins, the resulting metabolite, ammonia, may inhibit methane generation. Therefore, the effects of protein removal from disintegrated sludge on methane production were also studied. As a result, an obvious enhancement of biogas generation was observed by digesting disintegrated sludge (biogas yield increased from 15 to 36 ml/g CODadded·day for the raw excess sludge and the sonicated sludge, respectively). The quality of biogas was also improved by removing proteins from the disintegrated sludge. About 50% (w/w) of soluble proteins were removed from the suspension of disintegrated sludge by salting out using 35 g MgCl2·6H2O/l and also by isoelectric point precipitation at pH 3.3. For deproteinized sludge, methane production increased by 19%, and its yield increased from 145 ml/g CODremoved to 325 ml/g CODremoved. Therefore, the yield and quality of biogas produced from digestion of excess sludge can be enhanced by disintegrating the sludge and subsequent protein removal.
Similar content being viewed by others
Reference
B Atkinson F Macituna (1991) Biochemical Engineering and Biotechnology Handbook EditionNumber2 Stockton Press New York, NY
HB Choi KY Hwang EB Shin (1997) ArticleTitleA mechanical pretreatment of waste-activated sludge for H2S decrease on anaerobic digestion Biotechnol. Lett. 19 101–104 Occurrence Handle10.1023/A:1018387625297 Occurrence Handle1:CAS:528:DyaK2sXit1ahtr4%3D
C Gallert J Winter (1997) ArticleTitleMesophilic and thermophilic anaerobic digestion of source-sorted organic wastes: effects of ammonia on glucose degradation and methane production Appl. Microbiol. Biotechnol. 48 405–410 Occurrence Handle10.1007/s002530051071 Occurrence Handle1:CAS:528:DyaK2sXmsFartbo%3D
J Jung XH Xing K Matsumoto (2002) ArticleTitleRecoverability of protease released from disrupted excess sludge and its potential application to enhanced hydrolysis of protein in wastewater Biochem. Eng. J. 10 67–72 Occurrence Handle1:CAS:528:DC%2BD38XovFGrtA%3D%3D
J Kim C Park TH Kim Y Lee S Kim SW Kim J Lee (2003) ArticleTitleEffects of various pretreatments for enhanced anaerobic digestion with waste activated sludge J. Biosci. Bioeng. 95 271–275 Occurrence Handle1:CAS:528:DC%2BD3sXks1Kgsb0%3D Occurrence Handle16233404
JG Lin CN Chang SH Chang (1997) ArticleTitleEnhancement of anaerobic digestion of waste activated sludge by alkaline solubilization Bioresour. Technol. 62 85–90 Occurrence Handle10.1016/S0960-8524(97)00121-1 Occurrence Handle1:CAS:528:DyaK1cXlvVOitA%3D%3D
IW Nah YW Kang KY Hwang WK Song (2000) ArticleTitleMechanical pretreatment of wastewater activated sludge for anaerobic digestion process Water Res. 34 2362–2368 Occurrence Handle10.1016/S0043-1354(99)00361-9 Occurrence Handle1:CAS:528:DC%2BD3cXislynt7c%3D
R Navia M Soto G Vidal C Bornhard MC Diez (2002) ArticleTitleAlkaline pretreatment of kraft mill sludge to improve its anaerobic digestion Bull. Enviorn. Contam. Toxicol. 69 869–876 Occurrence Handle1:CAS:528:DC%2BD38XoslOrsbk%3D
HM Poggi-Varaldo R Rodíguez-Vázquez G Fernández-Villagómez F Esparaza-Gacía (1997) ArticleTitleInhibition of mesophilic solid-substrate anaerobic digestion by ammonia nitrogen Appl. Microbiol. Biotechnol. 47 284–291 Occurrence Handle10.1007/s002530050928 Occurrence Handle1:CAS:528:DyaK2sXitlequrk%3D
PG Stroot KD McMahon RI Mackie L Raskin (2001) ArticleTitleAnaerobic codigestion of municipal solid waste and biosolids under various mixing conditions-I. digester performance Water Res. 35 1804–1816 Occurrence Handle10.1016/S0043-1354(00)00439-5 Occurrence Handle1:CAS:528:DC%2BD3MXhvFWmsb0%3D Occurrence Handle11329683
A Tiehm K Nickel M Zellhorn U Neis (2001) ArticleTitleUltrasonic waste activated sludge disintegration for improving anaerobic stabilization Water Res. 35 2003–2009 Occurrence Handle10.1016/S0043-1354(00)00468-1 Occurrence Handle1:CAS:528:DC%2BD3MXisFCgtrw%3D Occurrence Handle11337847
M Weemaes H Grootaerd F Simoens W Verstraete (2000) ArticleTitleAnaerobic digestion of ozonized biosolids Water Res. 34 2330–2336 Occurrence Handle10.1016/S0043-1354(99)00373-5 Occurrence Handle1:CAS:528:DC%2BD3cXislynt7s%3D
HL Xu JY Wang JH Tay (2002) ArticleTitleA hybrid anaerobic solid-liquid bioreactor for food waste digestion Biotechnol. Lett. 24 757–761
Author information
Authors and Affiliations
Corresponding author
Additional information
Revisions requested 14 November 2005; Revisions received 13 January 2006
Rights and permissions
About this article
Cite this article
Cui, R., Jahng, D. Enhanced Methane Production from Anaerobic Digestion of Disintegrated and Deproteinized Excess Sludge. Biotechnol Lett 28, 531–538 (2006). https://doi.org/10.1007/s10529-006-0012-9
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10529-006-0012-9