Abstract
Various solvent extracts of the lichen Usnea ghattensis showed good antioxidant activity. A methanol extract prevented lipid peroxidation by 87% followed by 65% in Trolox at 20 μg/ml. It also showed superoxide anion scavenging activity and free radical scavenging activity 56% and 73%, respectively. The known antioxidants butylated hydroxytoluene (BHT), butylated hydroxyanisol (BHA) and quercetin at similar concentrations showed superoxide anion scavenging activity of 68, 59 and 47% and free radical scavenging activity 83, 77 and 69%, respectively. In addition, these extracts were inhibitory against Bacillus licheniformis, Bacillus megaterium, Bacillus subtilis and Staphylococcus aureus with MIC values of 5–10 μg/ml.
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References
MG Aiken (1977) Disinfection EA Rawlings (Eds) Bentley’s Textbook of Pharmaceutics EditionNumber8 Balliere Tindall London 498–526
BC Behera B Adawadkar U Makhija (2003) ArticleTitleInhibitory activity of xanthine oxidase and superoxide-scavenging activity in some taxa of the lichen family Graphidaceae Phytomedicine 10 536–543 Occurrence Handle10.1078/094471103322331511 Occurrence Handle13678240
BC Behera B Adawadkar U Makhija (2004) ArticleTitleCapacity of some Graphidaceous lichens to scavenge superoxide and inhibition of tyrosinase and xanthine oxidase activities Curr. Sci 87 83–87
MS Blois (1958) ArticleTitleAntioxidant determinations by the use of a stable free radical Nature 26 1199–1200
PD Crittenden N Porter (1991) ArticleTitleLichen forming fungi: potential sources of novel metabolites Trends Biotechnol 9 409–414 Occurrence Handle10.1016/0167-7799(91)90141-4 Occurrence Handle1367745
CF Culberson (1972) ArticleTitleImproved conditions and new data for the identification of lichen products by a standardized thin-layer chromatographic method J. Chromatogr 72 113–125 Occurrence Handle10.1016/0021-9673(72)80013-X Occurrence Handle5072880
PD Duh YY Tu GC Yen (1999) ArticleTitleAntioxidant activity of aqueous extract of harn jyur (Chyrsanthemum morifolium Ramat) Lebensm. Wiss. Technol 32 269–277 Occurrence Handle10.1006/fstl.1999.0548
European Pharmacopoeia (1971) Microbial assay of antibiotics. In: European Pharmacopoeia, Vol. 2. France: Maisonneuve S.A., pp. 49–52
HC Grice (1986) ArticleTitleSafety evaluation of butylated hydroxytoluene (BHT) in the liver, lung and gastro intestinal tract Food Chem. Toxicol 24 1127–1130 Occurrence Handle10.1016/0278-6915(86)90298-X Occurrence Handle3804115
T Hatano R Edamatsu A Mori Y Fujita E Yasuhara (1989) ArticleTitleEffect of interaction of tannins with co-existing substances. VI. Effects of tannins and related polyphenols on superoxide anion radical and on DPPH radical Chem. Pharm. Bull 37 2016–2021
C Liegeois G Lermusieau S Collins (2000) ArticleTitleMeasuring antioxidant efficiency of hort, malt and hops against the 2,2′-azobis (2-amidinopropane) dihydrochloride-induced oxidation of an aqueous dispersion of linoleic acid J. Agric. Food Chem 48 1129–1134 Occurrence Handle10.1021/jf9911242 Occurrence Handle10775361
NJ Miller AT Diplock CA Rice-Evans (1995) ArticleTitleEvaluation of the total antioxidant as a marker of the deterioration of apple juice on storage J. Agric. Food Chem 43 1794–1801 Occurrence Handle10.1021/jf00055a009
M Nishimiki NA Rao K Yagi (1972) ArticleTitleThe occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen Biochem. Biophys. Res. Commun 46 849–853 Occurrence Handle10.1016/S0006-291X(72)80218-3 Occurrence Handle4400444
JP Rauha S Remes M Heinonen A Hopia M Kahkonen T kujala K Pihlaja H Vuorela P Vuorela (2000) ArticleTitleAntimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds Int. J. Food Microbiol 56 3–12 Occurrence Handle10.1016/S0168-1605(00)00218-X Occurrence Handle10857921
C Sanchez-Moreno JA Larrauri F Saura-Calixto (1999) ArticleTitleFree radical scavenging capacity an inhibition of lipid oxidation of wines, grape juices and related polyphenolic constituents Food Res. Int 32 407–412 Occurrence Handle10.1016/S0963-9969(99)00097-6
K Slinkard VL Singleton (1977) ArticleTitleTotal phenol analyses: automation and comparison with manual methods Am. Enol. Vitic 28 49–55
HP Wichi (1988) ArticleTitleEnhanced tumor development by butylated hydroxyanisol (BHA) from the prospective of effect on fore stomach and oesophageal squamous epithelium Food Chem. Toxicol 26 717–723 Occurrence Handle10.1016/0278-6915(88)90072-5 Occurrence Handle3058561
Y Yamamoto Y Kinoshita H Matsubara K Kinoshita K Koyama K Takahashi T Kurokawa I Yoshimura (1998) ArticleTitleScreening of biological activities and isolation of biological active compounds from lichens Recent Res. Devel. Phytochem 2 23–34
Y Yamamoto R Mizuguchi Y Yamada (1985) ArticleTitleTissue cultures of Usnea rubescens and Ramalina yasudae and production of usnic acid in their cultures Agric. Biol. Chem 49 3347–3348
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Received after revisions 10 May 2005
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Behera, B., Verma, N., Sonone, A. et al. Antioxidant and antibacterial activities of lichen Usnea ghattensis in vitro. Biotechnol Lett 27, 991–995 (2005). https://doi.org/10.1007/s10529-005-7847-3
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DOI: https://doi.org/10.1007/s10529-005-7847-3