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Genome-wide Analysis of the Expression Profile of Saccharomyces cerevisiae in Response to Treatment with the Plant Isoflavone, Wighteone, as a Potential Antifungal Agent

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Abstract

The limitations of currently available antifungal agents and the rapid emergence of drug-resistant strains necessitate more efficient approaches to screening and developing novel antifungal drugs. The antifungal activity of the natural products of a series of plants was evaluated and wighteone, 5, 7, 4′–trihydroxy–6–(γ,γ-dimethylallyl)isoflavone showed excellent anti-yeast activity (MIC against Saccharomyces cerevisiae was 4 μg/ml). Transcriptome profiling of wighteone-treated S. cerevisiae indicated that wighteone is different from commonly used antifungal compounds in its mode of action.

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Authors and Affiliations

  1. Department of Biological Sciences and Biotechnology, Tsinghua University, 100084, Beijing, China

    Hongying Yin, Yongchao Zhao, Yan Zhang, Jing Cheng & Yuxiang Zhou

  2. Medical Systems Biology Research Center, School of Medicine, Tsinghua University, 100084, Beijing, China

    Hongying Yin, Yongchao Zhao, Yan Zhang, Jing Cheng & Yuxiang Zhou

  3. National Engineering Research Center for Beijing Biochip Technology (NERCBBT), 18 Life Science Parkway, 102206, Beijing, China

    Hongying Yin, Yongchao Zhao, Yan Zhang, Jing Cheng & Yuxiang Zhou

  4. Institute of Medicinal Plants Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 100094, Beijing, China

    Hongwu Zhang, Lizhen Xu & Zhongmei Zou

  5. CapitalBio Corporation, 18 Life Science Parkway, 102206, Beijing, China

    Weiping Yang

Authors
  1. Hongying Yin
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  2. Yongchao Zhao
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  3. Yan Zhang
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  4. Hongwu Zhang
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  5. Lizhen Xu
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  6. Zhongmei Zou
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  7. Weiping Yang
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  8. Jing Cheng
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  9. Yuxiang Zhou
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Corresponding author

Correspondence to Yuxiang Zhou.

Additional information

Received 17 June 2005; Revisions requested 4 July 2005 and 22 August 2005; Revisions received 8 August 2005 and 28 October 2005; Accepted 2 November 2005

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Yin, H., Zhao, Y., Zhang, Y. et al. Genome-wide Analysis of the Expression Profile of Saccharomyces cerevisiae in Response to Treatment with the Plant Isoflavone, Wighteone, as a Potential Antifungal Agent. Biotechnol Lett 28, 99–105 (2006). https://doi.org/10.1007/s10529-005-4954-0

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  • DOI: https://doi.org/10.1007/s10529-005-4954-0

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