Abstract
Nostoc muscorum, a heterocystous cyanobacterium, produced poly-β-hydroxybutyrate ( PHB) up to 8% (w/w) dry cells when grown photoautotrophically but 35% when grown mixotrophically with 0.4% (w/v) glucose and acetate after 21 d. Gas-exchange limitations under mixotrophy and chemoheterotrophy with 0.4% (w/v) acetate enhanced the accumulation up to 40–43% (w/w) dry cells, the value almost 5-fold higher with respect to photoautotrophic condition.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Dawes EA (1992) Storage polymers in prokaryotes. In: Mohan S, Dow C, Coles JA, eds. Prokaryotic Structure and Function: A New Perspective. Cambridge University Press, pp. 88–122.
IY Lee MK Kim HN Chang YH Park (1995) ArticleTitleRegulation of poly-beta-hydroxybutyrate biosynthesis by nicotinamide nucleotides in Alcaligenes eutrophus FEMS Microbiol. Lett. 131 35–39
M Miyake M Erata Y Asada (1996) ArticleTitleA thermophillic cyanobacterium, Synechococcus sp MA19, capable of accumulating poly-β-hydroxybutyrate. J. Ferment. Bioeng. 82 512–514
RA Pelroy GA Levine JA Basham (1976) ArticleTitleKinetics of light–dark CO2 fixation and glucose assimilation by Aphanocapsa 6714 J. Bacteriol. 128 633–643
V Riis W Mai (1988) ArticleTitleGas chromatographic determination of poly-β-hydroxybutyric acid in microbial biomass after hydrochloric acid propanolysis J. Chromatogr. 445 285–289
R Rippka J Deruelles JB Waterbury M Herdman RY Stanier (1979) ArticleTitleGeneric assignments, strain histories and properties of pure cultures of cyanobacteria J. Gen. Microbiol. 111 1–61
T Takahashi M Miyake Y Tokiwa Y Asada (1998) ArticleTitleImproved accumulation of poly-3-hydroxybutyrate by a recombinant cyanobacterium Biotechnol. Lett. 20 183–186
RJ Wegen Particlevan SY Lee AP Middelberg (2001) ArticleTitleMetabolic and kinetic analysis of poly(3-hydroxybutyrate) production by recombinant Escherichia coli Biotechnol. Bioeng. 74 70–80
M Vincenzini R De Philippis (1999) Polyhydroxyalkanoates Z. Cohen (Eds) Chemicals from Microalgae Taylor & Francis London 290–312
G Wu T Boa Z Shen Q Wu (2002) ArticleTitleSodium acetate stimulates PHB biosynthesis in Synechocystis sp PCC 6803. Tsing. Sci. Tech. 7 435–438
Author information
Authors and Affiliations
Corresponding author
Additional information
Revisions requested/10 September 2004; Revisions received 9 September 2004/11 November 2004
Rights and permissions
About this article
Cite this article
Sharma, L., Mallick, N. Enhancement of poly-β-hydroxybutyrate accumulation in Nostoc muscorum under mixotrophy, chemoheterotrophy and limitations of gas-exchange. Biotechnol Lett 27, 59–62 (2005). https://doi.org/10.1007/s10529-004-6586-1
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10529-004-6586-1