Abstract
Efficient secretion of human lysozyme from the yeast, Kluyveromyces lactis, was achieved by using more stable vectors in the order of S11 replication origin-containing episomal vector < full-length K. lactis plasmid pKD1-containing vector < centromeric vector < chromosome-integrated vectors. Cells containing a PGK (phosphoglycerate kinase) promoter-driven integration vector grown in non-selective rich medium achieved the highest level of secretion, ∼100 μg lysozyme secretion ml −1 culture: this level was ∼10-fold higher than that achieved by episomal vectors. An additional copy of the protein disulfide isomerase gene further facilitated the secretion.
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Iwata, T., Tanaka, R., Suetsugu, M. et al. Efficient secretion of human lysozyme from the yeast, Kluyveromyces lactis. Biotechnol Lett 26, 1803–1808 (2004). https://doi.org/10.1007/s10529-004-4614-9
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DOI: https://doi.org/10.1007/s10529-004-4614-9