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Taq1B Polymorphism of Cholesteryl Ester Transfer Protein (CETP) and Its Effects on the Serum Lipid Levels in Metabolic Syndrome Patients

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Abstract

The metabolic syndrome (MetS) is one of the most important risk factors for type 2 diabetes and cardiovascular disease. This syndrome is characterized by abdominal obesity, hypertension, insulin resistance, and dyslipidemia. The plasma origin of Cholesteryl ester transfer protein (CETP) is responsible for transferring cholesterol esters from high-density lipoprotein particles to apolipoprotein B containing lipoproteins compartment. We conducted this study to investigate the association between CETP gene Taq1B (rs708272) polymorphism in the metabolic syndrome among Iranian subjects. A sample size of 200 patients diagnosed with MetS together with 200 healthy donors as control were enrolled in this study. The investigation of polymorphism was performed by the use of polymerase chain reaction and restriction fragment length polymorphism analysis. To determine the relationship between polymorphism and lipid profile, we measured lipids and CETP concentration in metabolic syndrome and control subjects. Genotype distribution and allelic frequencies of polymorphism were determined and compared in both groups. Our findings showed that all clinical and biochemical characteristics in patients differed from the control group. The results showed that genotype and allele frequency of the Taq1B polymorphism was not significantly different between two groups. Instinctively, CETP was significantly higher in metabolic syndrome (1.64 ± 0.32 µg/ml) than in control (1.53 ± 0.34 µg/ml). A low level of CETP was found in blood of B2B2 typified genotype. In spite of Taq1B polymorphism on ester transfer protein concentration, no direct correlation was found between this polymorphism and metabolic syndrome.

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Authors and Affiliations

  1. Department of Clinical Biochemistry and Laboratory Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

    Nazila Fathi Maroufi

  2. Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran

    Khadijeh Farzaneh

  3. Solid Tumor Research Center, Urmia University of Medical Sciences, Urmia, Iran

    Mahdi Alibabrdel & Leila Zarei

  4. Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

    Omid Cheraghi & Sina Soltani

  5. Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St., Golgasht St., 5166614756, Tabriz, Iran

    Soheila Montazersaheb, Maryam Akbarzadeh & Mohammad Nouri

Authors
  1. Nazila Fathi Maroufi
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  2. Khadijeh Farzaneh
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  3. Mahdi Alibabrdel
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  4. Leila Zarei
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  5. Omid Cheraghi
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  7. Soheila Montazersaheb
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  8. Maryam Akbarzadeh
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  9. Mohammad Nouri
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Correspondence to Maryam Akbarzadeh or Mohammad Nouri.

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Maroufi, N.F., Farzaneh, K., Alibabrdel, M. et al. Taq1B Polymorphism of Cholesteryl Ester Transfer Protein (CETP) and Its Effects on the Serum Lipid Levels in Metabolic Syndrome Patients. Biochem Genet 54, 894–902 (2016). https://doi.org/10.1007/s10528-016-9766-5

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