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Cholesteryl ester transfer protein (CETP) −629C/A polymorphism and it,s effects on the serum lipid levels in metabolic syndrome patients

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Abstract

Metabolic syndrome is a relatively common disorder with significant morbidity worldwide. Cholesteryl ester transfer protein (CETP) plays a central role in the metabolism of lipoproteins. In this study the effect of −629C/A polymorphism on the concentration of CETP and plasma lipids pattern was elicited in metabolic syndrome patients and control subjects. For this, a sample of 200 patients diagnosed with metabolic syndrome disorder was studied in comparison with 200 healthy controls. This study was performed by using polymerase chain reaction and restriction fragment length polymorphisms. Genotype distribution and allelic frequencies were determined and compared in metabolic syndrome and healthy controls. To determine the relationship between −629C/A polymorphism and lipid levels, lipids and CETP concentration were measured in metabolic syndrome and normal subjects. The results showed a significant difference between two groups in terms of FBS, cholesterol, TG, HDL-C, LDL-C levels as well as BMI, waist circumference, systolic and diastolic blood pressure. The genotype frequencies for this polymorphism differed significantly between metabolic syndrome patients and controls (in control group: CC% 20.5, CA% 76, AA% 3.5 and in patient group: CC% 28.5, CA% 53.5, AA% 18) (p < 0.05) while there was no significant difference in the frequency of the alleles. In the two groups, the levels of the cholesteryl ester transfer protein in AA genotype were lower than other genotypes. In the control group, individuals with AA genotype had the highest levels of LDL-C and TC plasma concentration. Considering the results of this study, it can be concluded that the −629 AA genotype was associated with high cholesterol; high LDL-C and low CETP level, so that it can be related to metabolic syndrome.

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Acknowledgments

We are indebted to the Dey laboratory of Hamadan city for providing us with the samples. This study was financially supported by the Research Deputy of Hamadan University of Medical Sciences.

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Authors and Affiliations

  1. Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    T. Hassanzadeh & M. Saidijam

  2. Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    M. Akbarzadeh & T. Hassanzadeh

  3. Student’s Research Committee, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    R. Esmaeili

  4. Department of Endocrinology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Sh. Borzouei

  5. Department of Immunology, Hamadan University of Medical Sciences, Hamadan, Iran

    M. Hajilooi

  6. Department of Biostatistics, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran

    H. Mahjub

  7. School of Pharmacy and Center for Biomolecular Science, University of Nottingham, Nottingham, UK

    M. Paoli

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  1. M. Akbarzadeh
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  2. T. Hassanzadeh
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  3. M. Saidijam
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Correspondence to T. Hassanzadeh.

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Akbarzadeh, M., Hassanzadeh, T., Saidijam, M. et al. Cholesteryl ester transfer protein (CETP) −629C/A polymorphism and it,s effects on the serum lipid levels in metabolic syndrome patients. Mol Biol Rep 39, 9529–9534 (2012). https://doi.org/10.1007/s11033-012-1817-3

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  • DOI: https://doi.org/10.1007/s11033-012-1817-3

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