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Genetic Diversity and Differentiation in Hedychium spicatum, a Valuable Medicinal Plant of Indian Himalaya

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Abstract

Hedychium spicatum, a perennial rhizomatous medicinal plant distributed in subtropical and temperate parts, is considered nearly endemic to the Himalayan region.In this study allozyme markers were utilized to assess genetic variations and relationships among 12 distinct populations of this species from the West Himalaya of India. A high level of genetic diversity was found among the populations. Of the 13 loci generated using eight enzymes, 12 (92%) were polymorphic. F-statistics suggested a prevalence of a high heterozygote excess among populations (F IS = –0.51). Gene flow estimates and geographic distances between populations did not correlate significantly (r = –0.0258, P < 0.3550). The excess heterozygosity may be attributed to high pollinator mobility and inbreeding coefficient within the subpopulation, relative to the total F IS value. High frequencies of several alleles at different loci probably reflect the breeding pattern, as the species propagates clonally as well as through seeds. We also discuss conservation implications for the target species.

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Acknowledgments

The authors thank Dr. L. M. S. Palni, Director, GBPIHED, for providing the facilities and encouragement. Thanks are also due to Prof. Pedro Garcia, Universidad de Leon, Spain for the valuable input during experimental design and critical analysis of the data. UD thanks the National Academy of Sciences, India, and Hamdard University, New Delhi, for their support. Financial support from GBPIHED under Project 10 is gratefully acknowledged.

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Authors and Affiliations

  1. G. B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand, 263643, India

    Arun Jugran, Indra D. Bhatt, Sandeep Rawat, Lalit Giri & Ranbeer S. Rawal

  2. Department of Botany, Hamdard University (Jamia Hamdard), New Delhi, 110 062, India

    Uppeandra Dhar

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  1. Arun Jugran
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  2. Indra D. Bhatt
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  3. Sandeep Rawat
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Correspondence to Indra D. Bhatt.

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Jugran, A., Bhatt, I.D., Rawat, S. et al. Genetic Diversity and Differentiation in Hedychium spicatum, a Valuable Medicinal Plant of Indian Himalaya. Biochem Genet 49, 806–818 (2011). https://doi.org/10.1007/s10528-011-9451-7

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  • DOI: https://doi.org/10.1007/s10528-011-9451-7

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