Abstract
Much attention has been focused on the study of lactoferrin at the protein or nucleotide level in mice, humans, and cattle, but little is known about it in goats. The goat LF gene from 5′ UTR to exon 17 was amplified, and the variation of g.7605C→T in 10 Chinese indigenous goat breeds was analyzed. Among the three ruminant species (cattle, sheep, and goats), the intron–exon distribution pattern was similar, and all the exons had the same length, but the length of introns varied greatly due to insertions or deletions. The frequency of allele T at g.7605C→T (50.12%) was a little higher than that of allele C (49.88%), and the genotype distribution differed greatly between goat populations. The g.7605C→T site showed higher genetic diversity in goat populations. The genetic differentiation was 0.0783, and gene flow was 2.9433 among the 10 Chinese indigenous goat populations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baker EN, Rumball SV, Anderson BF (1987) Transferrins: insights into structure and function from studies on lactoferrin. Trends Biochem Sci 12:350–353
Connely OM (2001) Antiinflammatory activities of lactoferrin. J Am Coll Nutr 20:389–395
Fang P (2007) The relationship between Blf gene polymorphism and mastitis of dairy cows. Master’s Thesis, Northwest A&F University, Yangling, China
Levene H (1949) On a matching problem in genetics. Ann Math Stat 20:91–94
Li LH, Li XL, Zhou RY, Wang LZ (2008) Variation of goat MSHR gene G676A in different populations with different colors. Acta Vet Zootech Sin 39:429–436
Li XL, Valentini A (2004) Genetic diversity of Chinese indigenous goat breeds based on microsatellite markers. J Anim Breed Genet 121:350–355
Li XL, Zhang YP, Chen SO, Zeng FT, Qiu XP, Liu XM (2000) Study on the random amplified polymorphic DNA (RAPD) of main native goat breeds in China. Acta Vet Zootech Sin 31:416–422
Nei M (1972) Genetic distance between populations. Am Nat 106:283–292
Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70:3321–3323
Oztas YE, Ozgunes N (2005) Lactoferrin: a multifunctional protein. Adv Mol Med 1:149–154
Rodriguez DA, Vázquez L, Ramos G (2005) Antimicrobial mechanisms and potential clinical application of lactoferrin [in Spanish]. Rev Latinoam Microbiol l47:102–111
Shanbacher FL, Goodman RE, Talhouk RS (1992) Bovine mammary lactoferrin: implications from messenger ribonucleic acid (mRNA) sequence and regulation contrary to other milk proteins. J Dairy Sci 76:3812–3831
Shi Q (2007) Chimpanzee and human intron comparison reveals functional constraints and complex evolution. Master’s Thesis, Northwest A&F University, Yangling, China
Tang CJ, Zhou RY, Li XL (2008) Variation of 423G>T in the agouti gene exon 4 in indigenous Chinese goat breeds. Biochem Genet 46:770–780
Teng CT, Pentecost BT, Marshall A, Solomon A, Bowman BH, Lalley PA, Naylor SL (1987) Assignment of the lactotransferrin gene to human chromosome 3 and to mouse chromosome 9. Somat Cell Mol Genet 13:689–693
Vander SB, Belijaars L, Molema G, Harmsen MC, Meijer DK (2001) Antiviral activities of lactoferrin. Antiviral Res 52:225–239
Vogel HJ, Schibli D, Jing W, Lohmeier-Vogel EM, Epand RF, Epand RM (2002) Towards a structure function analysis of bovine lactoferricin and related tryptophan and arginine containing peptides. Biochem Cell Biol 80:49–63
Wang JT (2008) Study on RAB27A, the candidate gene of dilute coat color in goat. Master’s Thesis, Agricultural University of Hebei, Baoding, China
Acknowledgments
This study was funded by the subproject of the Breeding System Establishment Production in Scale of Dairy Cow with High Performance: Breeding System Establishment of Dairy Goat (No. 2006BAD04A10-06).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kang, JF., Li, XL., Zheng, HQ. et al. Sequence Analysis of the Lactoferrin Gene and Variation of g.7605C→T in 10 Chinese Indigenous Goat Breeds. Biochem Genet 49, 63–72 (2011). https://doi.org/10.1007/s10528-010-9385-5
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10528-010-9385-5