Skip to main content
SpringerLink
Log in

Genetic Polymorphisms of the CACNA2D1 Gene and Their Association with Carcass and Meat Quality Traits in Cattle

  • Published:
Biochemical Genetics Aims and scope Submit manuscript

Abstract

The objective of this study was to identify genetic polymorphisms of the CACNA2D1 gene and to analyze associations between SNPs and carcass and meat quality traits in cattle. Through PCR-RFLP and DNA sequencing methods, a new allelic variant corresponding to the A → G mutation (aspartic to glycine amino acid replacement) of the bovine CACNA2D1 gene was detected. Two alleles and three genotypes (AA, AG, and GG) were defined. Genetic character indicated that the A526745G locus showed moderate polymorphism and was in Hardy–Weinberg equilibrium. Gene-specific SNP marker association analysis showed that the A526745G mutant was significantly associated with carcass weight, dressing percentage, meat percentage, and backfat thickness. The results add new evidence that CACNA2D1 is an important candidate gene for the selection of carcass and meat quality traits in the cattle industry.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Andersson L, Haley CS, Ellegren H, Knott SA, Johansson M, Andersson K, Andersson-Eklund L, Edfords-Lilja I, Fredholm M, Hansson I, Hakansson J, Lundstrom K (1994) Genetic mapping of quantitative trait loci for growth and fatness in pigs. Science 263:1771–1774

    Article  CAS  PubMed  Google Scholar 

  • Ashwell MS, Heyen DW, Weller JI, Ron M, Sonstegard TS, Van Tassell CP, Lewin HA (2005) Detection of quantitative trait loci influencing conformation traits and calving ease in Holstein–Friesian cattle. J Dairy Sci 88:4111–4119

    Article  CAS  PubMed  Google Scholar 

  • Buitkamp J, Ewald D, Masabanda J, Bishop MD, Fries R (2003) FISH and RH mapping of the bovine alpha (2)/delta calcium channel subunit gene (CACNA2D1). Anim Genet 34:302–318

    Article  Google Scholar 

  • Casas E, Stone RT, Keele JW, Shackelford SD, Kappes SM, Koohmaraie M (2001) A comprehensive search for quantitative trait loci affecting growth and carcass composition of cattle segregating alternative forms of the myostatin gene. J Anim Sci 79:854–860

    CAS  PubMed  Google Scholar 

  • Fujii J, Otsu K, Zorzato F, Leon SD, Khanna VK, Weiler JE, O’Brien SD, Maclennan DH (1991) Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthermia. Science 253:448–451

    Article  CAS  PubMed  Google Scholar 

  • Gutiérrez-Gil B, Williams JL, Homer D, Burton D, Haley CS, Wiener P (2009) Search for quantitative trait loci affecting growth and carcass traits in a cross population of beef and dairy cattle. J Anim Sci 87:24–36

    Article  PubMed  Google Scholar 

  • Li JH, Chu XH, Guo XL, Xu NY (2005) Radiation hybrid mapping of skeletal muscle calcium channel genes CACNB1 and CACNG1 to porcine chromosome 12 and CACNA2D1 to porcine chromosome 9. Anim Genet 36:358–359

    Article  CAS  PubMed  Google Scholar 

  • Li JH, Yang MS, Xu NY (2007) Single nucleotide polymorphism in 3′ UTR of porcine CACNA2D1 gene and its genetic effect on meat quality trait. Acta Agric Boreali Sin 22:76–79

    Google Scholar 

  • Li JH, Yang MS, Xu NY (2008) Mapping of porcine CACNA2D1 gene and the integrative analysis of correlative genetic maps. J Northwest A&F Univ (Nat Sci Ed) 36:49–53

    Google Scholar 

  • Loke J, Maclennan DH (1998) Malignant hyperthermia and central core disease: disorders of Ca2+ release channels. Am J Med 104:470–486

    Article  CAS  PubMed  Google Scholar 

  • Longeri M, Polli M, Strillacci MG, Samore AB, Zanotti M (2006) Short communication: quantitative trait loci affecting the somatic cell score on chromosomes 4 and 26 in Italian Holstein cattle. J Dairy Sci 89:3175–3177

    Article  CAS  PubMed  Google Scholar 

  • Mizoshita K, Watanabe T, Hayashi H, Kubota C, Yamakuchi H, Todoroki J, Sugimoto Y (2004) Quantitative trait loci analysis for growth and carcass traits in a half-sib family of purebred Japanese Black (Wagyu) cattle. J Anim Sci 82:3415–3420

    CAS  PubMed  Google Scholar 

  • Mullenbach R, Lagoda PJ, Welter C (1989) An efficient salt-chloroform extraction of DNA from blood and tissues. Trends Genet 5:391

    CAS  PubMed  Google Scholar 

  • NAGRP (2010) A snapshot of QTL on cattle chromosome 4. Cattle QTLdb, NAGRP, Bioinformatics Coordination Program. http://www.animalgenome.org/cgi-bin/QTLdb

  • Noguera JL, Varona L, Gómez-Raya L, Sánchez A, Babot D, Estany J, Messer LA, Rothschild M, Pérez-Enciso M (2003) Estrogen receptor polymorphism in Landrace pigs and its association with litter size performance. Livest Prod Sci 2:53–59

    Article  Google Scholar 

  • Robinson RL, Curran JL, Ellis FR, Halsall PJ, Hall WJ, Hopkins PM, Iles DE, West SP, Shaw M-A (2000) Multiple interacting gene products may influence susceptibility to malignant hyperthermia. Ann Hum Genet 64:307–320

    Article  CAS  PubMed  Google Scholar 

  • Robinson RL, Brooks C, Brown SL, Ellis FR, Halsall PJ, Quinnell RJ, Shaw MA, Hopkins PM (2002) RYR1 mutations causing central core disease are associated with more severe malignant hyperthermia in vitro contracture test phenotypes. Hum Mutat 20:88–97

    Article  CAS  PubMed  Google Scholar 

  • Rupp R, Boichard D (2003) Genetics of resistance to mastitis in dairy cattle. Vet Res 34:671–688

    Article  PubMed  Google Scholar 

  • Sherman EL, Nkrumah J, Li C, Bartusiak R, Murdoch B, Moore SS (2009) Fine mapping quantitative trait loci for feed intake and feed efficiency in beef cattle. J Anim Sci 87:37–45

    Article  CAS  PubMed  Google Scholar 

  • Stone RT, Casas E, Smith TP, Keele JW, Harhay G, Bennett GL, Koohmaraie M, Wheeler TL, Shackelford SD, Snelling WM (2005) Identification of genetic markers for fat deposition and meat tenderness on bovine chromosome 5: development of a low-density single nucleotide polymorphism map. J Anim Sci 83:2280–2288

    CAS  PubMed  Google Scholar 

  • Takasuga A, Watanabe T, Mizoguchi Y, Hirano T, Ihara N, Takano A, Yokouchi K, Fujikawa A, Chiba K, Kobayashi N, Tatsuda K, Oe T, Furukawa-Kuroiwa M, Nishimura-Abe A, Fujita T, Inoue K, Mizoshita K, Ogino A, Sugimoto Y (2007) Identification of bovine QTL for growth and carcass traits in Japanese Black cattle by replication and identical-by-descent mapping. Mamm Genome 18:125–136

    Article  PubMed  Google Scholar 

  • Yeh FC, Yang RC, Boyle TBJ, Ye ZH, Mao JX (1997) Popgene, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton, Canada (http://www.ualberta.ca/*fyeh/info.htm)

  • Yokouchi K, Mizoguchi Y, Watanabe T, Iwamoto E, Sugimoto Y, Takasuga A (2009) Identification of a 3.7-Mb region for a marbling QTL on bovine chromosome 4 by identical-by-descent and association analysis. Anim Genet 40:945–951

    Article  CAS  PubMed  Google Scholar 

  • Zhang Q, Boichard D, Hoeschele I, Ernst C, Eggen A, Murkve B, Pfister-Genskow M, Witte LA, Grignola FE, Uimari P, Thaller G, Bishop MD (1998) Mapping quantitative trait loci for milk production and health of dairy cattle in a large outbred pedigree. Genetics 149:1959–1973

    CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This research was financially supported by the earmarked fund for Modern Agro-industry Technology Research System (No. nycytx-38), the New Variety of Transgenic Organism Great Breeding Program (No. 2008ZX08007-002 and No. 2009ZX08009-157B), the Eleventh “Five-Year” National Science and Technology Support Project (No. 2006BAD04A16 and No. 2007BAD56B04), the National 863 Program of China (No. 2008AA10Z146), and the Public-welfare Profession Research Program (No. nyhyzx07-035-07).

Author information

Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China

    Guan-Yu Hou

  2. Laboratory of Molecular Biology and Bovine Breeding, Key Laboratory for Farm Animal Genetic Resources and Utilization, Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China

    Guan-Yu Hou, Zheng-Rong Yuan, Xue Gao, Jun-Ya Li, Hui-Jiang Gao, Jin-Bao Chen & Shang-Zhong Xu

Authors
  1. Guan-Yu Hou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zheng-Rong Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xue Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jun-Ya Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hui-Jiang Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jin-Bao Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Shang-Zhong Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shang-Zhong Xu.

Additional information

The first two authors have contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hou, GY., Yuan, ZR., Gao, X. et al. Genetic Polymorphisms of the CACNA2D1 Gene and Their Association with Carcass and Meat Quality Traits in Cattle. Biochem Genet 48, 751–759 (2010). https://doi.org/10.1007/s10528-010-9357-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10528-010-9357-9

Keywords

Search

Navigation