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Analysis of Diversity in Chinese Cultivated Barley with Simple Sequence Repeats: Differences Between Eco-Geographic Populations

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Abstract

The genetic diversity of 116 barley accessions, representing five Chinese eco-geographic populations, was studied using simple sequence repeat (SSR) markers. The 21 SSR loci revealed 128 alleles with an average of 6.1 alleles per locus. The highest values of proportion of polymorphic loci (P) and gene diversity index (He) were obtained in the Northern (P = 1.00; He = 0.60) and the Yangtze River reaches and Southern populations (P = 1.00; He = 0.59). The lowest values were in the populations of the Yellow River reaches (P = 0.86; He = 0.44). The highest average number of alleles per locus (4.52) and number of unique alleles (7) were found in the Qinghai–Tibet plateau population. Cluster analysis revealed that together with the row type, strong eco-geographic variables influenced the classification. Associations of SSR and eco-geographic values were established for 11 SSR loci. Four to six markers were found to discriminate among geographic groups, which may serve as tools for diagnosis of the eco-geographic populations and provide evidence for the adaptive nature of SSR markers.

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Acknowledgments

We thank Prof. Sun Lijun for kindly providing seeds of barley accessions. The research was supported by the Key Program of the Natural Science Foundation of Tianjin (Grant No. 07JCZDJC03800), the Scientific Research Foundation for the Returned Overseas Chinese Scholars of the Ministry of Education (Grant No. 2003-14).

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Authors and Affiliations

  1. Lab of Molecular Genetics, College of Life Sciences, Nankai University, 300071, Tianjin, China

    Feixue Chen, Defu Chen, Zhen Gao & Xiwen Chen

  2. Departamento de Genética y Producción Vegetal, Estación Experimental de Aula Dei, CSIC, Apartado 13034, 50059, Zaragoza, Spain

    María-Pilar Vallés

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  1. Feixue Chen
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  2. Defu Chen
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  3. María-Pilar Vallés
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  4. Zhen Gao
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  5. Xiwen Chen
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Correspondence to Xiwen Chen.

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Chen, F., Chen, D., Vallés, MP. et al. Analysis of Diversity in Chinese Cultivated Barley with Simple Sequence Repeats: Differences Between Eco-Geographic Populations. Biochem Genet 48, 44–56 (2010). https://doi.org/10.1007/s10528-009-9294-7

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  • DOI: https://doi.org/10.1007/s10528-009-9294-7

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