Skip to main content
SpringerLink
Log in

Assessing Genetic Diversity of Chinese Cultivated Barley by STS Markers

  • Published:
Genetic Resources and Crop Evolution Aims and scope Submit manuscript

Abstract

To assess the genetic diversity among China’s cultivated barley, sequence tagged site (STS) marker analysis was carried out to characterize 109 morphologically distinctive accessions originating from five Chinese eco-geographical zones. Fourteen polymorphic STS markers representing at least one in each chromosome were chosen for the analysis. The 14 STS markers revealed a total of 47 alleles, with an average of 3.36 alleles per locus (range 2–8). The proportion of polymorphic loci per population averaged 0.84 (range 0.71–1.00); the mean gene diversity averaged 0.39 (range 0.28–0.49). The means of P and H e were highest in the Yangtze reaches and Southern zone (P = 1.00; H e = 0.46) and lowest (P = 0.71 He = 0.28) in the Yellow river reaches zone. The STS diversity in different zones is quite different from the morphology diversity. The STS variation was partitioned into 17% among the zone and 83% within the zone. Both cluster and principal coordinate analyses clearly separated the accessions into a dispersed group (mostly two-rowed barley with a lower mean GS value) and a concentrated group (mostly six-rowed barley with a higher mean GS value) according to the spike characteristic with only a few exceptions. The accessions from the Qinghai-Tibet plateau formed a distinctive subgroup and can be distinguished from the concentrated group. The role of Tibet in the origin and evolution of cultivated barley has been discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aldrich P.R., Doebley J., Schertz K.F. and Stec A. (1992). Theor. Appl. Genet. 85: 293–302

    Google Scholar 

  • Bekeke E. (1983). A differential rate of regional distribution of barley flavonoid patterns in Ethiopiaand a view on the center of origin of barley. Hereditas 98: 269–280

    Google Scholar 

  • Chen H.B., Marin J.M., Lavin M. and Talbert L.E. (1994). Genetic diversity in hard red spring wheat based on sequence-tagged-site PCR markers. Crop Sci. 34: 1628–1632

    Article  CAS  Google Scholar 

  • Cole C.G., Goodfellow P.N., Bobrow M. and Bentley D.R. (1991). Generation of novel sequence tagged sites (STSs) from discrete chromosomal zones using Alu-PCR. Genomics 10: 816–826

    Article  CAS  PubMed  Google Scholar 

  • Guo H., Wei Y.M., Chen F. and Zhang Y.L. (2002). Genetic diversity of Hordeum bogdanii wilensky native to Xinjiang, Chinabased on STS-PCR markers. Acta Botanica Sinica 44: 1327–1332

    CAS  Google Scholar 

  • Hatz B.G., Jahoor A. and Fischbeck G. 1996. RFLP-polymorphism among European accessions of the barley core collection. Proc. VII Int. Barley Genetics SymposiumSaskatoon, pp. 176–178.

  • Kempthorne O. (1955). The randomization theory of experimental inference. J. Am. Stat. Assoc. 50: 946–967

    Article  Google Scholar 

  • Komatsuda T., Nakamura I., Takaiwa F. and Oka S. (1998). Development of STS markers closely linked to the vrs1 locus in barley, Hordeum vulgare. Genome 41: 680–685

    Article  CAS  Google Scholar 

  • Liu F., Sun G.L., Salomon B. and Bothmer R. (2001). Distribution of allozymic alleles and genetic diversity in the American Barley Core Collection. Theor. Appl. Genet. 102: 606–615

    Article  CAS  Google Scholar 

  • Liu F., Salomon B. and Bothmer R. (2000). Genetic diversity in European accessions of the Barley Core Collection as detected by isozyme electrophoresis. Genet. Res. Crop. Evo. 47: 571–581

    Article  CAS  Google Scholar 

  • Mano Y., Sayed-Tabatabaei B.E., Graner A., Blake T., Takaiwa F. and Komatsuda T. (1999). Map construction of sequence-tagged sites (STSs) in barley (Hordeum vulgare L.). Theor. Appl. Genet. 98: 937–946

    Article  CAS  Google Scholar 

  • Melchinger A.E., Graner A., Singh M. and Messmer M.M. (1994). Relationships among European barley germplasm. I. Genetic diversity among winter and spring cultivars revealed by RFLPs. Crop Sci. 34: 1191–1199

    Article  Google Scholar 

  • Mullis K.B. and Faloona F.A. (1987). Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 155: 335–350

    Article  CAS  PubMed  Google Scholar 

  • Nei M. (1973). Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA 70: 3321–3323

    Article  CAS  PubMed  Google Scholar 

  • Nei M. (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583–590

    PubMed  CAS  Google Scholar 

  • Nei M. and Li W.H. (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76: 5269–5273

    Article  CAS  PubMed  Google Scholar 

  • Olson M., Hood L., Cantor C. and Doststein D. (1989). A common language for physical mapping of the human genome. Science 245: 1434–1435

    Article  CAS  PubMed  Google Scholar 

  • Saghai-Maroof M.A., Soliman K.M., Jorgensen R.A. and Allard R.W. (1984). Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritancechromosomal location, and population dynamics. Proc. Natl. Acad. Sci. USA 81: 8014–8018

    Article  CAS  PubMed  Google Scholar 

  • Saghai-Maroof M.A., Zhang Q.F. and Biyashev R.M. (1995). Comparison of restriction fragment length polymorphisms (RFLPs) in wild and cultivated barley. Genome 38: 298–306

    Google Scholar 

  • Saghai-Maroof M.A., Zhang Q.F. and Chojecki J. (1994). RFLPs in cultivated barley and their application in the evaluation of malting quality cultivars. Hereditas 121: 21–29

    Article  Google Scholar 

  • Struss D. and Plieske J. (1998). The use of microsatellite markers for detection of genetic diversity in barley populations. Theor. Appl. Genet. 97: 308–315

    Article  CAS  Google Scholar 

  • Sun L.J. (1988). Varieties of cultivated barley and their distribution in China. Chin. Agric. Sci. 2: 25–31 (in Chinese with English summary)

    Google Scholar 

  • Sun L.J. (2001). Chinese Barley Genetic Resources and Elite Germplasm. Chinese Agricultural Scientific and Technological Publishers, Beijing (in Chinese)

    Google Scholar 

  • Sun L.J., Gao J.Y. and Guan J.P. (1995). Genetic diversity and geographic distribution in the Chinese barley germplasm based on esterase isozyme. Crop Germplasm 2: 1–5 (in Chinese)

    Google Scholar 

  • Sun L.J., Liu W., Zhang J. and Zhang W.X. (1999). Investigation of barley germplasm in China. Genet. Res. Crop. Evol. 46: 361–369

    Article  Google Scholar 

  • Talbert L.E., Blake N.K., Chee P.W., Blake T.K. and Magyar G.M. (1994). Evaluation of ‘sequence-tagged-site’ PCR products as molecular markers in wheat. Theor. Appl. Genet. 87: 789–794

    Article  CAS  Google Scholar 

  • Tragoonrung S., Kanazin V., Hayes P.M. and Blake T.K. (1992). Sequence-tagged-site facilitated PCR for barley genome mapping. Theor. Appl. Genet. 84: 1002–1008

    Article  CAS  Google Scholar 

  • Weir B.S. (1990). Genetic Data Analysis: Methods for Discrete Population Genetic Data. Sinauer Assoc. Inc., Sunderland, MA, USA

    Google Scholar 

  • Zhang J. and Cao Y.S. (1999). Population structure and phenotypic diversity of barley in China’s gene bank. Sci. Agric. Sin 32: 20–26 (in Chinese with English abstract)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Life Sciences, Nankai University, Tianjin, 300071, China

    Xiwen Chen, Shaoying Guo, Defu Chen, Pin Liu & Xiangdong Jia

  2. Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Science, 100859, Beijing, China

    Lijun Sun

Authors
  1. Xiwen Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shaoying Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Defu Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pin Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiangdong Jia
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lijun Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xiwen Chen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, X., Guo, S., Chen, D. et al. Assessing Genetic Diversity of Chinese Cultivated Barley by STS Markers. Genet Resour Crop Evol 53, 1665–1673 (2006). https://doi.org/10.1007/s10722-005-1289-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10722-005-1289-6

Keywords

Search

Navigation