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Isolation and Characterization of the Cetn1 Gene from Tufted Deer (Elaphodus cephalophus)

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Abstract

The tufted deer, a species found only in China, has polymorphic sex chromosomes (2n = 46, 47, 48). Centrins are centrosome components in species from yeast to humans. Here, the Cetn1 gene was isolated from the testis cDNA library of tufted deer, and its expression pattern, number of gene copies, and gene structure were studied. To date, for unknown reasons, satisfactory genomic DNA of the tufted deer has not been obtained. In the present study, we extracted high molecular weight genomic DNA from tissues of the tufted deer. Results showed that genomic DNA was isolated successfully and could be used for Southern blotting of the Cetn1 gene. Centrin 1 transcripts were shown to exist in testis and the retina. It has no intron and only a single copy in the genome. Protein sequence analyses indicate that the C-terminal Ser-170, which was shown to be phosphorylated in human centrin, is not conserved except in sequences from the human, chimpanzee, and tufted deer.

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Acknowledgments

We thank Mr. Chunmao Xu and Mr. Jun Hu (Wannan First-Aid Center for Wild Animals, Anhui Province, China) for providing tufted deer samples. This study was supported by the Key Project of the Shanghai Science and Technology Committee of China (No. 063919121) and the Chinese National Natural Science Foundation (No. 30370789).

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Authors and Affiliations

  1. School of Agriculture and Biology, Shanghai JiaoTong University, 800 Dongchuan Road, Shanghai, 200240, P.R. China

    Wen Zhang & Xiuguo Hua

  2. School of Life Science, Nanjing Normal University, 1 Wenyuan Road, Nanjing, 210046, P.R. China

    Xiangrong Cao, Quan Shen, Wei Liu & Xiran Zhang

  3. Institute of Medical Biotechnology, Chinese Academy of Medical Science, 2 Nanwei Road, Beijing, 100050, P.R. China

    Siyang Ni

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  1. Wen Zhang
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  2. Xiangrong Cao
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  3. Quan Shen
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  4. Wei Liu
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  5. Siyang Ni
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  6. Xiuguo Hua
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  7. Xiran Zhang
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Correspondence to Xiuguo Hua.

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Wen Zhang and Xiangrong Cao contributed equally to this work.

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Zhang, W., Cao, X., Shen, Q. et al. Isolation and Characterization of the Cetn1 Gene from Tufted Deer (Elaphodus cephalophus). Biochem Genet 46, 652–662 (2008). https://doi.org/10.1007/s10528-008-9179-1

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  • DOI: https://doi.org/10.1007/s10528-008-9179-1

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