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Molecular cloning, mapping, and tissue expression of the porcine cluster of differentiation 14 (CD14) gene

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Abstract

CD14 plays an important role in initiating the innate response to lipopolysaccharide from Gram-negative bacteria. The gene and corresponding cDNA of porcine CD14 were sequenced and characterized. The porcine CD14 gene consists of two exons and a short intron (80 bp) located immediately after the ATG translation start codon. This structure is very similar to the CD14 gene of human, rat, mouse, rabbit, horse, and cow. The sequence of the porcine CD14 protein is 59–76% identical to that of rat, mouse, rabbit, human, horse, and cow CD14 protein. A highly conserved structure of the CD14 protein with respect to the leucine-rich repeats domain and the N-glycosylation sites was observed between species. Porcine CD14 was assigned to porcine chromosome 2q21 by a radiation hybrid panel. Using RT-PCR analysis, porcine CD14 transcripts were detected in liver, spleen, thymus, white matter, and skeletal muscle.

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References

  • Akira S, Uematsu S, Takeuchi O (2006) Pathogen recognition and innate immunity. Cell 124:783–801

    Article  PubMed  CAS  Google Scholar 

  • Antal-Szalmas P (2000) Evaluation of CD14 in host defence. Eur J Clin Invest 30:167–179

    Article  PubMed  CAS  Google Scholar 

  • Arroyo-Espliguero R, El-Sharnouby K, Vazquez-Rey E, Kalidas K, Jeffery S, Kaski JC (2005) CD14 C(-260)T promoter polymorphism and prevalence of acute coronary syndromes. Int J Cardiol 98:307–312

    Article  PubMed  Google Scholar 

  • Bendtsen JD, Nielsen H, von Heijne G, Brunak S (2004) Improved prediction of signal peptides: SignalP 3.0. J Mol Biol 340:783–795

    Article  PubMed  CAS  Google Scholar 

  • Beutler B, Rietschel ET (2003) Innate immune sensing and its roots: the story of endotoxin. Nat Rev Immunol 3:169–176

    Article  PubMed  CAS  Google Scholar 

  • Bochkov VN, Kadl A, Huber J, Gruber F, Binder BR, Leitinger N (2002) Protective role of phospholipid oxidation products in endotoxin-induced tissue damage. Nature 419:77–81

    Article  PubMed  CAS  Google Scholar 

  • Cho K, Pham TN, Crivello SD, Jeong J, Green TL, Greenhalgh DG (2004) Involvement of CD14 and toll-like receptor 4 in the acute phase response of serum amyloid A proteins and serum amyloid P component in the liver after burn injury. Shock 21:144–150

    Article  PubMed  CAS  Google Scholar 

  • Donati M, Berglundh T, Hytonen AM, Hahn-Zoric M, Hanson LA, Padyukov L (2005) Association of the -159 CD14 gene polymorphism and lack of association of the -308 TNFA and Q551R IL-4RA polymorphisms with severe chronic periodontitis in Swedish Caucasians. J Clin Periodontol 32:474–479

    Article  PubMed  CAS  Google Scholar 

  • Ferrero E, Goyert SM (1988) Nucleotide sequence of the gene encoding the monocyte differentiation antigen, CD14. Nucleic Acids Res 16:4173

    Article  PubMed  CAS  Google Scholar 

  • Ferrero E, Hsieh CL, Francke U, Goyert SM (1990) CD14 is a member of the family of leucine-rich proteins and is encoded by a gene syntenic with multiple receptor genes. J Immunol 145:331–336

    PubMed  CAS  Google Scholar 

  • Gao PS, Mao XQ, Baldini M, Roberts MH, Adra CN, Shirakawa T, Holt PG, Martinez FD, Hopkin JM (1999) Serum total IgE levels and CD14 on chromosome 5q31. Clin Genet 56:164–165

    Article  PubMed  CAS  Google Scholar 

  • Garcia CA, Prabakar KR, Diez J, Cao ZA, Allende G, Zeller M, Dogra R, Mendez A, Rosenkranz E, Dahl U, Ricordi C, Hanahan D, Pugliese A (2005) Dendritic cells in human thymus and periphery display a proinsulin epitope in a transcription-dependent, capture-independent fashion. J Immunol 175:2111–2122

    PubMed  CAS  Google Scholar 

  • Ikeda A, Takata M, Taniguchi T, Sekikawa K (1997) Molecular cloning of bovine CD14 gene. J Vet Med Sci 59:715–719

    Article  PubMed  CAS  Google Scholar 

  • Jensen LJ, Gupta R, Blom N, Devos D, Tamames J, Kesmir C, Nielsen H, Staerfeldt HH, Rapacki K, Workman C, Andersen CA, Knudsen S, Krogh A, Valencia A, Brunak S (2002) Prediction of human protein function from post-translational modifications and localization features. J Mol Biol 319:1257–1265

    Article  PubMed  CAS  Google Scholar 

  • Kajava AV, Kobe B (2002) Assessment of the ability to model proteins with leucine-rich repeats in light of the latest structural information. Protein Sci 11:1082–1090

    Article  PubMed  CAS  Google Scholar 

  • Kobe B, Kajava AV (2001) The leucine-rich repeat as a protein recognition motif. Curr Opin Struct Biol 11:725–732

    Article  PubMed  CAS  Google Scholar 

  • Kruger S, Graf J, Merx MW, Stickel T, Koch KC, Janssens U, Hanrath P, Kunz D (2005) CD14 gene -260 C/T polymorphism is associated with chronic heart failure. Eur J Intern Med 16:345–347

    Article  PubMed  CAS  Google Scholar 

  • Lee JD, Kato K, Tobias PS, Kirkland TN, Ulevitch RJ (1992) Transfection of CD14 into 70Z/3 cells dramatically enhances the sensitivity to complexes of lipopolysaccharide (LPS) and LPS binding protein. J Exp Med 175:1697–1705

    Article  PubMed  CAS  Google Scholar 

  • Leung TF, Tang NL, Wong GW, Fok TF (2005) CD14 and toll-like receptors: potential contribution of genetic factors and mechanisms to inflammation and allergy. Curr Drug Targets Inflamm Allergy 4:169–175

    Article  PubMed  CAS  Google Scholar 

  • LeVan TD, Bloom JW, Bailey TJ, Karp CL, Halonen M, Martinez FD, Vercelli D (2001) A common single nucleotide polymorphism in the CD14 promoter decreases the affinity of Sp protein binding and enhances transcriptional activity. J Immunol 167:5838–5844

    PubMed  CAS  Google Scholar 

  • Liu S, Shapiro RA, Nie S, Zhu D, Vodovotz Y, Billiar TR (2000) Characterization of rat CD14 promoter and its regulation by transcription factors AP1 and Sp family proteins in hepatocytes. Gene 250:137–147

    Article  PubMed  CAS  Google Scholar 

  • Liu Y, Walter S, Stagi M, Cherny D, Letiembre M, Schulz-Schaeffer W, Heine H, Penke B, Neumann H, Fassbender K (2005) LPS receptor (CD14): a receptor for phagocytosis of Alzheimer’s amyloid peptide. Brain 128:1778–1789

    Article  PubMed  Google Scholar 

  • Lloyd KL, Kubes P (2006) GPI linked endothelial CD14 contributes to the detection of LPS. Am J Physiol Heart Circ Physiol

  • Lund O, Nielsen M, Lundegaard C, Worning P (2002) CPHmodels 2.0: X3M a computer program to extract 3D Models. In Abstract at the CASP5 conference, p. A102

  • Marmey B, Boix C, Barbaroux JB, Dieu-Nosjean MC, Diebold J, Audouin J, Fridman WH, Mueller CG, Molina TJ (2006) CD14 and CD169 expression in human lymph nodes and spleen: specific expansion of CD14+CD169- monocyte-derived cells in diffuse large B-cell lymphomas. Hum Pathol 37:68–77

    Article  PubMed  CAS  Google Scholar 

  • Medzhitov R, Janeway C Jr (2000) Innate immunity. N Engl J Med 343:338–344

    Article  PubMed  CAS  Google Scholar 

  • Milan D, Hawken R, Cabau C, Leroux S, Genet C, Lahbib Y, Tosser G, Robic A, Hatey F, Alexander L, Beattie C, Schook L, Yerle M, Gellin J (2000) IMpRH server: an RH mapping server available on the Web. Bioinformatics 16:558–559

    Article  PubMed  CAS  Google Scholar 

  • Pasquier LD (2006) Germline and somatic diversification of immune recognition elements in Metazoa. Immunol Lett 104:2–17

    Article  PubMed  CAS  Google Scholar 

  • Rettenberger G, Bruch J, Fries R, Archibald AL, Hameister H (1996) Assignment of 19 porcine type I loci by somatic cell hybrid analysis detects new regions of conserved synteny between human and pig. Mamm Genome 7:275–279

    Article  PubMed  CAS  Google Scholar 

  • Shih HH, Lin TM, Chuang JH, Eng HL, Juo SH, Huang FC, Chen CL, Chen HL (2005) Promoter polymorphism of the CD14 endotoxin receptor gene is associated with biliary atresia and idiopathic neonatal cholestasis. Pediatrics 116:437–441

    Article  PubMed  Google Scholar 

  • Su GL, Dorko K, Strom SC, Nussler AK, Wang SC (1999) CD14 expression and production by human hepatocytes. J Hepatol 31:435–442

    Article  PubMed  CAS  Google Scholar 

  • Sutherland GR, Baker E, Callen DF, Hyland VJ, Wong G, Clark S, Jones SS, Eglinton LK., Shannon MF., Lopez AF (1988) Interleukin 4 is at 5q31 and interleukin 6 is at 7p15. Hum Genet 79:335–337

    Article  PubMed  CAS  Google Scholar 

  • Van Gucht S, Van Reeth K, Nauwynck H, Pensaert M (2005) Porcine reproductive and respiratory syndrome virus infection increases CD14 expression and lipopolysaccharide-binding protein in the lungs of pigs. Viral Immunol 18:116–126

    Article  PubMed  Google Scholar 

  • Vychodilova-Krenkova L, Matiasovic J, Horin P (2005) Single nucleotide polymorphisms in four functionally related immune response genes in the horse: CD14,TLR4, Cepsilon, and Fcepsilon R1 alpha. Int J Immunogenet 32:277–283

    Article  PubMed  CAS  Google Scholar 

  • Wright SD, Ramos RA, Tobias PS, Ulevitch RJ, Mathison JC (1990) CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science 249:1431–1433

    Article  PubMed  CAS  Google Scholar 

  • Yerle M, Pinton P, Robic A, Alfonso A, Palvadeau Y, Delcros C, Hawken R, Alexander L, Beattie C, Schook L, Milan D, Gellin J (1998) Construction of a whole-genome radiation hybrid panel for high-resolution gene mapping in pigs. Cytogenet Cell Genet 82:182–188

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This study was supported by the Key project of the National Natural Science Foundation of China (Grant No. 30430500) and the National Key Basic Research Program (Grant No. 2006CB102104). We also gratefully acknowledge M. Yerle (INRA, Toulouse) for the pig radiation hybrid (IMpRH) panels.

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Authors and Affiliations

  1. Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, P.R. China

    Xiao-Tian Qiu, Yu-Hua Li, HeJun Li, Ying Yu & Qin Zhang

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  1. Xiao-Tian Qiu
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  2. Yu-Hua Li
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  3. HeJun Li
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  4. Ying Yu
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  5. Qin Zhang
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Correspondence to Qin Zhang.

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Qiu, XT., Li, YH., Li, H. et al. Molecular cloning, mapping, and tissue expression of the porcine cluster of differentiation 14 (CD14) gene. Biochem Genet 45, 459–468 (2007). https://doi.org/10.1007/s10528-007-9088-8

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  • DOI: https://doi.org/10.1007/s10528-007-9088-8

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