Abstract
Among other positive effects, mild stresses can increase resistance to severe stresses. Previous studies combining two mild stresses showed that when each mild stress had positive effects their combination had more positive effects than each mild stress. The present study tested whether combining three mild stresses could still provide positive additive effects, or whether this combination has negative effects because it is no longer a mild stress but rather a strong stress with negative effects. Flies were subjected to either fasting, hypergravity for one or two weeks, or cold shocks, to combinations of two or of the three mild stresses, and survival to a severe cold stress was observed at 13 or 20 days of age. Positive effects of each mild stress and of combining two stresses could be observed, but combining the three stresses provided a similar survival or a lower survival than the combination of two stresses. Thus, combining three stresses was not more efficient than combining two stresses.
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Calabrese EJ, Dhawan G, Kapoor R, Iavicoli I, Calabrese V (2015) What is hormesis and its relevance to healthy aging and longevity? Biogerontology 16:693–707
Gomez FH, Sambucetti P, Norry FM (2016) Elevated extension of longevity by cyclically heat stressing a set of recombinant inbred lines of Drosophila melanogaster throughout their adult life. Biogerontology 17:883–892
Henten AVM, Loeschcke V, Pedersen JG, Leisner JJ, Sarup P (2016) Injuries can prolong lifespan in Drosophila melanogaster males. Biogerontology 17:337–346
Kim H, Suzuki T, Saito K, Kim M, Kojima N, Ishizaki T, Yamashiro Y, Hosoi E, Yoshida H (2013) Effectiveness of exercise with or without thermal therapy for community-dwelling elderly Japanese women with non-specific knee pain: a randomized controlled trial. Arch Gerontol Geriatr 57:352–359
Le Bourg E (2012) Combined effects of two mild stresses (cold and hypergravity) on longevity, behavioral aging, and resistance to severe stresses in Drosophila melanogaster. Biogerontology 13:313–328
Le Bourg E (2013) Fasting can protect young and middle-aged Drosophila melanogaster flies against a severe cold stress. Biogerontology 14:513–529
Le Bourg E (2015) Fasting and other mild stresses with hormetic effects in Drosophila melanogaster can additively increase resistance to cold. Biogerontology 16:517–527
Le Bourg E (2016) Life-time protection against severe heat stress by exposing young Drosophila melanogaster flies to a mild cold stress. Biogerontology 17:409–415
Le Bourg E, Massou I (2015) Fasting increases survival to cold in FOXO, DIF, autophagy mutants and in other genotypes of Drosophila melanogaster. Biogerontology 16:411–421
Le Bourg E, Rattan SIS (eds) (2008) Mild stress and healthy aging. Applying hormesis in aging research and interventions. Springer, Berlin
Le Bourg E, Minois N, Bullens P, Baret P (2000) A mild stress due to hypergravity exposure at young age increases longevity in Drosophila melanogaster males. Biogerontology 1:145–155
Lints FA, Bullens P, Le Bourg E (1993) Hypergravity and aging in Drosophila melanogaster. 7. New longevity data. Exp Geront 28:611–615
Mattson MP, Calabrese EJ (eds) (2010) Hormesis. A revolution in biology, toxicology and medicine. Springer, Dordrecht
McClure CD, Zhong W, Hunt VL, Chapman FM, Hill FV, Priest NK (2014) Hormesis results in trade-offs with immunity. Evolution 68:2225–2233
Rattan SIS, Le Bourg E (eds) (2014) Hormesis in health and disease. CRC Press, Boca Raton
Sarup P, Loeschcke V (2011) Life extension and the position of the hormetic zone depends on sex and genetic background in Drosophila melanogaster. Biogerontology 12:109–117
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Le Bourg, É. Combining three mild stresses in Drosophila melanogaster flies does not have a more positive effect on resistance to a severe cold stress than combining two mild stresses. Biogerontology 18, 275–284 (2017). https://doi.org/10.1007/s10522-017-9689-5
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DOI: https://doi.org/10.1007/s10522-017-9689-5