Abstract
5-Methylcytosine (m5C) has a plethora of functions and roles in various biological processes including human diseases and aging. A TLC-based fast and simple method for quantitative determination of total genomic levels of m5C in DNA is described, which can be applicable to aging research with respect to rapid and high throughput screening and comparison. Using this method, an example of the analysis of global alternations of m5C in serially passaged human skin fibroblasts is provided, which shows age-related global hypomethylation during cellular aging in vitro. This method can be useful for screening potential modulators of aging at the level of epigenetic alterations.
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Acknowledgements
This work was supported within the project of MNISZW to M.B. Laboratory of Cellular Ageing at the University of Aarhus, Denmark is supported by research grants from the Danish Medical Research Council (FSS).
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Barciszewska, M.Z., Barciszewska, A.M. & Rattan, S.I.S. TLC-based detection of methylated cytosine: application to aging epigenetics. Biogerontology 8, 673–678 (2007). https://doi.org/10.1007/s10522-007-9109-3
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DOI: https://doi.org/10.1007/s10522-007-9109-3