The possibility of finding persistent SARS-CoV-2 viral particles in human peripheral blood leukocytes after a novel coronavirus infection was shown. The results of droplet digital PCR showed that 19 of 24 examined subjects had from 4 to 555 copies of the Nsp4 SARS-CoV-2 gene in 5-6 months after infection. The presence of this transcript in peripheral blood leukocytes was associated with reduced expression of FOXP3 gene and increased level of RORγ gene mRNA. The copy number of the Nsp4 gene negatively correlated with the level of FOXP3 gene mRNA (r=-0.45; p=0.028), but showed a positive correlation with the DANCR long non-coding RNA (r=0.94; p<0.001). In SARS-CoV-2-positive healthy individuals, the level of TLR2, NLRP3, and IL1B gene transcripts was higher than in SARS-CoV-2-negative donors. The presence of SARS-CoV-2 in a persistent form is probably associated with impaired immunosuppression and the development of chronic inflammation in apparently healthy volunteers after a new coronavirus infection.
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Li S, Bai L, Dong J, Sun R, Lan K. Kaposi’s sarcoma-associated herpesvirus: epidemiology and molecular biology. Adv. Exp. Med. Biol. 2017;1018:91-127. https://doi.org/10.1007/978-981-10-5765-6_7
Bevova MR, Netesov SV, Aulchenko YS. The new coronavirus COVID-19 infection. Mol. Gen. Microbiol. Virol. 2020;35(2):53-60. https://doi.org/10.3103/S0891416820020044
Zhang L, Richards A, Barrasa MI, Hughes SH, Young RA, Jaenisch R. Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues. Proc. Natl. Acad. Sci. USA. 2021;118(21):e2105968118. https://doi.org/10.1073/pnas.2105968118
Easterbrook JD, Zink MC, Klein SL. Regulatory T cells enhance persistence of the zoonotic pathogen Seoul virus in its reservoir host. Proc. Natl Acad. Sci. USA. 2007;104(39):15 502-15 507. https://doi.org/10.1073/pnas.0707453104
Jiang M, Guo Y, Luo Q, Huang Z, Zhao R, Liu S, Le A, Li J, Wan L. T-Cell Subset counts in peripheral blood can be used as discriminatory biomarkers for diagnosis and severity prediction of Coronavirus disease 2019. J. Infect. Dis. 2020;222(2):198-202. https://doi.org/10.1093/infdis/jiaa252
Drago F, Ciccarese G, Rebora A, Parodi A. Human herpesvirus-6, -7, and Epstein-Barr virus reactivation in pityriasis rosea during COVID-19. J. Med. Virol. 2021;93(4):1850-1851. https://doi.org/10.1002/jmv.26549
Pinto Lde F, Compri CM, Fornari JV, Bartchewsky W, Cintra DE, Trevisan M, Carvalho Pde O, Ribeiro ML, Velloso LA, Saad MJ, Pedrazzoli JJr, Gambero A. The immunosuppressant drug, thalidomide, improves hepatic alterations induced by a high-fat diet in mice. Liver Int. 2010;30(4):603-610. https://doi.org/10.1111/j.1478-3231.2009.02200.x
Huang H, Lu Z, Jiang C, Liu J, Wang Y, Xu Z. Imbalance between Th17 and regulatory T-Cells in sarcoidosis. Int. J. Mol. Sci. 2013;14(11):21 463-21 473. https://doi.org/10.3390/ijms141121463
Gadanec LK, McSweeney KR, Qaradakhi T, Ali B, Zulli A, Apostolopoulos V. Can SARS-CoV-2 virus use multiple receptors to enter host cells? Int. J. Mol. Sci. 2021;22(3):992. https://doi.org/10.3390/ijms22030992
Laha S, Saha C, Dutta S, Basu M, Chatterjee R, Ghosh S, Bhattacharyya NP. In silico analysis of altered expression of long non-coding RNA in SARS-CoV-2 infected cells and their possible regulation by STAT1, STAT3 and interferon regulatory factors. Heliyon. 2021;7(3):e06395. https://doi.org/10.1016/j.heliyon.2021.e06395
Zhang X, Ma L, Zhang C, Hou B, Zhou Y, Yu S. Silencing LncRNA-DANCR attenuates inflammation and DSS-induced endothelial injury through miR-125b-5p. Gastroenterol. Hepatol. 2021;44(9):644-653. https://doi.org/10.1016/j.gastrohep.2020.10.008
Xie C, Guo Y, Lou S. LncRNA ANCR promotes invasion and migration of gastric cancer by regulating FoxO1 expression to inhibit macrophage M1 polarization. Dig. Dis. Sci. 2020;65(10):2863-2872. https://doi.org/10.1007/s10620-019-06019-1
Qian W, Cai X, Qian Q, Wang D, Zhang L. Angelica sinensis polysaccharide suppresses epithelial-mesenchymal transition and pulmonary fibrosis via a DANCR/AUF-1/FOXO3 regulatory axis. Aging Dis. 2020;11(1):17-30. https://doi.org/10.14336/AD.2019.0512
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 173, No. 6, pp. 726-731, June, 2022
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Topchieva, L.V., Balan, O.V., Men’shenin, A.V. et al. Quantitative Assay of SARS-CoV-2 RNA and Level of Proinflammatory Protein Gene Transcripts in Peripheral Blood Leukocytes after a Novel Coronavirus Infection. Bull Exp Biol Med 173, 740–744 (2022). https://doi.org/10.1007/s10517-022-05621-0
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DOI: https://doi.org/10.1007/s10517-022-05621-0