Culturing of bone marrow cells in serum-free RPMI-1640 medium for 24 h was accompanied by a decrease in the rate of [3H]-thymidine incorporation into DNA. Addition of native apolipoprotein A-I (apoA-I) or plasma LDL and HDL to the culture medium increased this parameter. In contrast to native apoA-I, its modified form decelerated DNA synthesis in bone marrow cells. A similar inhibitory effect of modified protein was observed in cultures of human embryonic kidney cells (HEK293) and in rapidly proliferating mouse macrophage cell line ANA-1. The only exclusion was human myeloid cell line U937: neither native nor modified apoA-I affected DNA synthesis in these cells. Thus, the regulatory effects of apoA-I are tissue-specific; this protein can produce either stimulatory or inhibitory effect on DNA biosynthesis in cells depending on its conformation.
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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 155-159, July, 2017
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Usynin, I.F., Dudarev, A.N., Miroshnichenko, S.M. et al. Effect of Native and Modified Apolipoprotein A-I on DNA Synthesis in Cultures of Different Cells. Bull Exp Biol Med 164, 247–251 (2017). https://doi.org/10.1007/s10517-017-3967-8
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DOI: https://doi.org/10.1007/s10517-017-3967-8