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Effect of Factors Secreted by the Placenta on Phenotype of THP-1 Cells Cultured on a 3D Scaffold

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Bulletin of Experimental Biology and Medicine Aims and scope

We studied the effects of secretory products of the placenta obtained from women with normal pregnancy and preeclampsia on the expression of surface markers by THP-1 cells cultured on a 3D Matrigel scaffold. Secretory products of third trimester placentas obtained from women with normal pregnancy reduced the relative number of THP-1 cells expressing CD54 and CD14 molecules and expression of CD14 and CD95 molecules by THP-1 cells in comparison with the effect of secretory products first trimester placentas. In parallel, the intensity of CD49d expression by THP-1 cells increased in the presence of secretory products of third trimester placentas in comparison with the first trimester. No differences in the expression of the studied molecules by THP-1 cells under the effect of placentas from women with physiological pregnancy and patients with preeclampsia were found.

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Authors and Affiliations

  1. D. O. Ott Reseach Institute of Obstetrics and Gynecology, St. Petersburg, Russia

    T. Yu. Lvova, O. I. Stepanova, L. P. Viazmina, L. S. Okorokova, K. L. Belyakova, M. E. Belikova, S. A. Selkov & D. I. Sokolov

Authors
  1. T. Yu. Lvova
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  2. O. I. Stepanova
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  3. L. P. Viazmina
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  4. L. S. Okorokova
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  5. K. L. Belyakova
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  6. M. E. Belikova
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  7. S. A. Selkov
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  8. D. I. Sokolov
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Corresponding author

Correspondence to D. I. Sokolov.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 26-32, January, 2016

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Lvova, T.Y., Stepanova, O.I., Viazmina, L.P. et al. Effect of Factors Secreted by the Placenta on Phenotype of THP-1 Cells Cultured on a 3D Scaffold. Bull Exp Biol Med 161, 162–167 (2016). https://doi.org/10.1007/s10517-016-3368-4

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  • DOI: https://doi.org/10.1007/s10517-016-3368-4

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