Supplementation of the ration with eicosapentaenoic and docosahexaenoic ω-3 polyunsaturated fatty acids (PUFA) in doses of 0.3 and 1 g/kg body weight for 4 weeks had no effect on ethoxyresorufin O-dealkylase (EROD) activity and expression of the CYP1A1 gene in male Wistar rats, but caused a dose-dependent increase in methoxyresorufin O-dealkylase (MROD) activity of CYP1A2 (by 28 and 73%, respectively) without significant changes in CYP1A2 mRNA expression. ω-3 PUFA had no effect on the indole-3-carbinol-induced (20 mg/kg body weight over the last 7 days of the experiment) EROD activity and expression of CYP1A1 mRNA. The indole-3-carbinol-induced MROD activity was shown to increase by 6.2 times in rats not receiving ω-3 PUFA and only by 3.9 and 2.7 times in animals receiving ω-3 PUFA. The indole-3-carbinol-induced expression of CYP1A2 mRNA slightly increased in animals receiving ω-3 PUFA. Our results suggest that the effect of ω-3 PUFA on the induced and basal activity of CYP1A2 is not related to modulation of CYP1A2 gene expression.
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References
L. V. Kravchenko, I. V. Aksenov, N. V. Trusov, et al., Vopr. Pitaniya, 81, No. 1, 24-29 (2012).
O. N. Mikhailova, M. L. Filippenko, O. A. Timofeeva, et al., Biomed. Zh., 49, No. 4, 388-393 (2003).
A. V. Pogozheva, Cardiovascular Diseases, Diet, and ω-3 PUFA [in Russian], Moscow (2000).
V. A. Tutel’yan, N. V. Trusov, G. V. Guseva, et al., Bull. Exp. Biol. Med., 154, No. 2, 250-254 (2012).
J. Adams, D. Sibbritt, C. W. Lui, et al., BMJ Open, 3, No. 4, e002292 (2013).
S. J. Carlson, E. M. Fallon, B. T. Kalish, et al., J. Parenter. Enteral. Nutr., 37, No. 1, 15-22 (2013).
R. S. Chapkin, D. N. McMurray, L. A. Davidson, et al., Brit. J. Nutr., 100, No. 6, 1152-1157 (2008).
M. Fer, Y. Dreano, D. Lucas, et al., Arch. Biochem. Biophys., 471, No. 2, 116-125 (2008).
S. Kanji, D. Seely, F. Yazdi, et al., Syst. Rev., 1, 26 (2012).
C. C. Li, C. K. Lii, K. L. Liu, et al., Toxicol. Appl. Pharmacol., 225, No. 3, 329-336 (2007).
D. W. Nebert, T. P. Dalton, A. B. Okey, and F. J. Gonzalez, J. Biol. Chem., 279, No. 23, 23,847-23,850 (2004).
A. Parkinson, Casarett & Doull’s Toxicology: The Basic Science of Poisons, Ed. C. D. Klaassen, 6th Ed., New York (2001), p. 133-224.
M. Saito, Ann. Nutr. Metab., 38, No. 6, 313-321 (1994).
S. Schmidt, F. Stahl, K. O. Mutz, et al., Nutr. Metab. (London), 9, No. 1, 45 (2012).
H. T. Yao, Y. W. Chang, S. J. Lan, et al., Life Sci., 79, No. 26, 2432-2440 (2006).
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 156, No. 9, pp. 302-306, September, 2013
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Kravchenko, L.V., Tutel’yan, V.A., Trusov, N.V. et al. Effect of Polyunsaturated Fatty Acids ω-3 on the Induction of Activity and Expression of CYP1A1 and CYP1A2 Genes in the Liver of Rats under the Influence of Indole-3-Carbinol. Bull Exp Biol Med 156, 327–331 (2014). https://doi.org/10.1007/s10517-014-2341-3
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DOI: https://doi.org/10.1007/s10517-014-2341-3