We described a spectrophotometric method for measuring hemoglobin peroxidase activity in human plasma using o-dianisidine (o-DA) as the substrate and myeloperoxidase specific inhibitor 4-aminobensoic acid hydrazide (ruling out the probable contribution of myeloperoxidase to the measured parameter value). The optimal conditions (pH 5.5; 2 mM H2O2) have been determined, at which hemoglobin makes the main contribution to plasma oxidation of o-DA. A signifi cant positive correlation between hemoglobin peroxidase activity measured by the spectrophotometric method and hemoglobin level measured by the pyridine hemochromogenic method has been detected (r=0.624; p<0.01) in plasma specimens from 16 donors. Plasma hemoglobin peroxidase activities were measured in healthy individuals and patients with type 2 diabetes mellitus and coronary heart disease. High plasma hemoglobin peroxidase activities in both groups of patients indicates disorders in the mechanisms of clearance of hemoglobin and its highly reactive derivatives and can serve as specifi c markers of diseases associated with oxidative stress.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
L. A. Blumenfeld, Sorosovsk. Obrazovat. Zh., No. 4, 33-34 (1998).
I. V. Gorudko, O. S. Cherkalina, A. V. Sokolov, et al., Bioorgan. Khim., 35, No. 5, 1-11 (2009).
Information and Methodological Aid, Ed. V. I. Pupkova [in Russian], Koltsovo (2011).
O. V. Kosmachevskaya and A. F. Topunov, Prikladn. Biokhim. Mikrobiol., 45, No. 6, 627-653 (2009).
O. V. Kosmachevskaya and A. F. Topunov, Ibid., 43, No. 3, 347-353 (2007).
Z. I. Mikashinovich, E. V. Olempieva, R. A. Gridasova, and A. A. Shevtsov, Kubansk. Nauch. Med. Vestn., Nos. 3-4, 131-134 (2010).
Human Physiology, Eds. V. M. Pokrovsky et al. [in Russian], Moscow (2003).
Guidelines for Laboratory Physicians for Hemoglobin Measurements, Eds. A. A. Kozlov et al. [in Russian], Moscow (2008).
N. P. Chesnokova, E. V. Ponukalina, and M. N. Bizenkova, Uspekhi Sovr. Estestvoznan., No. 7, 29-34 (2006).
A. Kapralov, I. I. Vlasova, W. Feng, et al., J. Biol. Chem., 284, No. 44, 30,395-30,407 (2009).
W. H. Waugh, J. Pediatr. Hematol. Oncol., 25, No. 10, 831-834 (2003).
C. Zhang, J. Yang, and L. K. Jennings, Diabetes, 53, No. 11, 2950-2959 (2004).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 155, No. 1, pp. 129-132, January, 2013
Rights and permissions
About this article
Cite this article
Grigorieva, D.V., Gorudko, I.V., Sokolov, A.V. et al. Measurement of Plasma Hemoglobin Peroxidase Activity. Bull Exp Biol Med 155, 118–121 (2013). https://doi.org/10.1007/s10517-013-2095-3
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-013-2095-3