Multipotent characteristics of human fetal (9-11.5 weeks) pigmented epithelial retinal cells and capacity to transdifferentiation in neuronal direction were studied in vitro under different culturing conditions. The cultures were analyzed using a wide spectrum of antibodies. It was found that pigmented epithelium of human eye is a heterogeneous cell population with three subtypes differing by adhesion characteristics, migration, transdifferentiation potential, and reaction to microenvironmental factors. Subtype 1 cells steadily retain their epithelial characteristics, subtype 2 cells change their morphotype and produce neuroblast and photoreceptor cell proteins, and subtype 3 cells form free fl oating spheres and are capable to multipotent differentiation.
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Translated from Kletochnye Tehnologii v Biologii i Medicine, No. 3, pp. 128-135, July, 2009
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Milyushina, L.A., Poltavtseva, R.A., Marei, M.V. et al. In Vitro Phenotypic Modifi cation of Pigmented Epithelium Cells from Human Eye at Early Stages of Development. Bull Exp Biol Med 148, 113–119 (2009). https://doi.org/10.1007/s10517-009-0657-1
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DOI: https://doi.org/10.1007/s10517-009-0657-1