We developed a new approach to evaluation of the intensity of osteogenic differentiation of mesenchymal stromal stem cells based on measurement of optical density of mesenchymal stromal cell cultures in a concentration range 625–10,000 cells per well after their culturing in osteogenic induction medium and staining of calcium deposits by the method of von Kossa. The proposed method allows comparative semiquantitative evaluation of osteogenic properties of mesenchymal stem cells depending on tissue sources (bone marrow, adipose tissue, placenta), in vitro cell density, number of passages, duration of culturing, and concentration of serum growth factors in the microenvironment. The developed approach makes it possible to compare functional activity of mesenchymal stromal cells in various pathologies. The proposed method can be used in traumatology and orthopedics for improving the efficiency of transplantation of mesenchymal stromal cells for stimulation of reparative osteogenesis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
M. S. Rostovskaya, S. Z. Sharifullina, N. I. Chupikova, and A. S. Teplyashin, Thitologiya, 46, No. 10, 937 (2004).
P. S. Anker, S. A. Scherjon, C. Kleijburg-van der Keur, et al., Blood, 102, 1548–1549 (2003).
P. S. Anker, S. A. Scherjon, C. Kleijburg-van der Keur, et al., Stem Cells, 22, 1338–1345 (2004).
M. A. Baxter, R. F. Wynn, S. N. Jowitt, et al., Ibid., 22, No. 5, 675–682 (2004).
M. M. Bonab, K. Alimoghaddam, F. Talebian, et al., BMC Cell Biology, 7, 14–20 (2006).
S. L. Cheng, J. W. Yang, L. Rifas, et al., Endocrinology, 134, 277–286 (1994).
G. D’Ippolito, S. Diabira, G.A. Howard, et al., J. Cell Sci., 117, 2971–2981 (2004).
M. Dominici, K. Le Blanc, I. Mueller, et al., Cytotherapy, 8, No. 4, 315–317 (2006).
A. Erices, P. Conget, and J. J. Minguell, Br. J. Hematol., 109, 235–242 (2000).
A. J. Friedenstein , R. K. Chailakhyan, N. V. Latsinik, et al., Transplantation, 17, 331–340 (1974).
Y. Fukuchi, H. Nakajima, D. Sugiyama, et al., Stem Cells, 22, 649–658 (2004).
C. A. Gregory, W. G. Gunn, A. Peister, and D. J. Prockop, Analyt. Biochem., 329, No. 1, 77–84 (2004).
S. Gronthos, A. C. W. Zannettino, S.J. Hay, et al., J. Cell Sci., 116, 1827–1835 (2003).
E. M. Horwitz, D.J. Prockop, L. A. Fitzpatrick, et al., Nat. Med., 5, 309–313 (1999).
N. Jaiswal, S. E. Haynesworth, A. I. Caplan, and S. P. Bruder, J. Cell. Biochem., 64, 295–312 (1997).
S. Kern, H. Eichler, J. Stoeve, et al., Stem Cells, 24, 1294–1301 (2006).
N. Ogura, M. Kawada, W. J. Chang, et al., J. Oral Science, 46, No. 4, 207–213 (2004).
M. F. Pittenger, A. M. Mackay, S. C. Beck, et al., Science, 284, 143–147 (1999).
I. Sekiya, B. Larson, J. R. Smith, et al., Stem Cells, 20, 530–541 (2002).
B. L. Yen, H. I. Huang, C. C. Chien, et al., Ibid., 23, 3–9 (2005).
P. A. Zuk, M. Zhu, P. Ashjian, et al., Mol. Biol. Cell., 13, No. 12, 4279–4295 (2002).
P. A. Zuk, M. Zhu, H. Mizuno, et al., Tissue Eng., 7, 211–228 (2001).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Kletochnye Tehnologii v Biologii i Medicine, No. 4, pp. 219–225, November, 2008
Rights and permissions
About this article
Cite this article
Ostanin, A.A., Petrovskiy, Y.L., Shevela, E.Y. et al. A New Approach to Evaluation of Osteogenic Potential of Mesenchymal Stromal Cells. Bull Exp Biol Med 146, 534–539 (2008). https://doi.org/10.1007/s10517-009-0321-9
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-009-0321-9