Abstract
We evaluated possible therapeutic effect of multipotent mesenchymal stromal cells from human adipose tissue differentiated to neuronal phenotype with retinoic acid on Wistar rats subjected to toxic effect of 3-nitropropionic acid. Transplantation of mesenchymal stromal cells from human adipose tissue considerably decreased neurological symptoms, normalized exploratory activity (open field test) and long-term memory (Morris test), which correlated with normalization of pathomorphological manifestations in the brain. Destructive changes in the caudate nucleus caused by treatment with 3-nitropropionic acid (reduced size of neurons, changes in their shape, and cell edema) tended to decrease under the effect of multipotent mesenchymal stromal cells: the area of neurons increased 2-fold, the cells acquired typical round shape, cell edema decreased.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Microscopic Technique: A Manual, Ed. D. S. Sarkisov and Yu. L. Petrov [in Russian], Moscow (1996).
R. M. Hudoerkov, E. L. Dovedova, and D. N. Voronkov, Byull. Eksp. Biol. Med., 144, No. 7, 39–41 (2007).
T. Alexi, P. E. Hughes, R. L. Faull, and C. E. Williams, Neuroreport., 9, No. 11, R57–R64 (1998).
A. Bjorklund and O. Lindvall, Nat. Neurosci., 3, No. 6, 537–544 (2000).
Blum D., Galas M.C., Gall D. et al., Neurobiol. Dis., 10, No. 3, 410–426 (2002).
M. S. Guyot, P. Hantraye, R. Dolan, et al., Neuroscience, 79, No. 1, 45–56 (1997).
S. K. Kang, L. A. Putnam, J. Y. Ylostalo, et al., J. Cell Sci., 117, 4289–4299 (2004).
Kokai L.E., Rubin J.P., Marra K.G. Plast. Reconstr. Surg., 116, No. 5, 1453–1460 (2005).
A. Kulikov, A. Eva, U. Kirch, A. Boldyrev, G. Scheiner-Bobis, Biochim. Biophys. Acta., 1768, No. 7, 1691–1702 (2007).
R. G. Morris, P. Garrud, J. N. Rawlins, and J. O’Keefe, Nature, 297, No. 5868, 681–683 (1982).
Planat-Benard V., Silvestre J.S., Cousin B., et al., Circulation, 109, No. 5, 656–663 (2004).
Rosser A.E., Zietlow R., and Dunnett S.B., Curr. Opin. Neurol., 20, No. 6, 688–692 (2007).
Ryu J.K., Kim J., Cho S.J., et al., Neurobiol. Dis., 16, No. 1, 68–77 (2004).
Safford K.M. and Rice H.E., Curr. Drug Targets., 6, No. 1, 57–62 (2005).
P. A. Zuk, M. Zhu, P. Ashjian, et al., Mol. Biol. Cell., 13, No. 12, 4279–4295 (2002).
Author information
Authors and Affiliations
Additional information
__________
Translated from Kletochnye Tehnologii v Biologii i Meditsine, No. 2, pp. 83–89, April, 2008
Rights and permissions
About this article
Cite this article
Kulikov, A.V., Stepanova, M.S., Stvolinsky, S.L. et al. Application of multipotent mesenchymal stromal cells from human adipose tissue for compensation of neurological deficiency induced by 3-nitropropionic acid in rats. Bull Exp Biol Med 145, 514–519 (2008). https://doi.org/10.1007/s10517-008-0131-5
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-008-0131-5