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Structure and cell composition of spheres cultured from human fetal retina

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

The structure and cell composition of spheres obtained by culturing human fetal retinal cells after 15, 18, 22–23, and 24 weeks of gestation were studied. The cells were cultured as neurospheres: in serum-free medium with growth factors, in which they formed floating spheres. Immunocytochemical analysis showed that cell proliferation in the spheres decreased with increasing fetal age. Stem/progenitor cells, neuroblasts, and photoreceptors were detected in the spheres. Glial cells were detected only in spheres originating from 22-and 24-week fetuses. All spheres, irrespective of age and duration of culturing, consisted of numerous cell rosettes, each histotypically similar to the neuroblastic layer of the developing retina.

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Authors and Affiliations

  1. N. K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Russia

    M. A. Aleksandrova, O. V. Podgornyi, R. A. Poltavtseva & I. G. Panova

  2. Center of Obstetrics, Gynecology, and Perinatology, Russian Academy of Medical Sciences, Moscow

    G. T. Sukhikh

Authors
  1. M. A. Aleksandrova
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  2. O. V. Podgornyi
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  3. R. A. Poltavtseva
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  4. I. G. Panova
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  5. G. T. Sukhikh
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Corresponding author

Correspondence to M. A. Aleksandrova.

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__________

Translated from Kletochnye Tekhnologii v Biologii i Medicine, No. 3, pp. 171–179, August, 2006

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Aleksandrova, M.A., Podgornyi, O.V., Poltavtseva, R.A. et al. Structure and cell composition of spheres cultured from human fetal retina. Bull Exp Biol Med 142, 152–159 (2006). https://doi.org/10.1007/s10517-006-0315-9

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  • DOI: https://doi.org/10.1007/s10517-006-0315-9

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