Abstract
The propagation of neural precursors in culture is an essential tool for the study of the signaling matrix that regulates their proliferation, self-renewal, and generation of terminally differentiated progeny. Neural precursors can be expanded in vitro using both adherent and non-adherent culture protocols. The culture of fetal human neural precursors in the absence of serum as free-floating clusters of cells termed neurospheres is described here.
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Acknowledgment
This work was supported by a Multiple Sclerosis Society of Canada Grant to A.C. and S.W., and a Canadian Institutes of Health Research grant to S.W.
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Chojnacki, A., Weiss, S. (2013). Culturing Fetal Precursor Cells Using Free Floating Serum-Free Conditions. In: Reynolds, B., Deleyrolle, L. (eds) Neural Progenitor Cells. Methods in Molecular Biology, vol 1059. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-574-3_1
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DOI: https://doi.org/10.1007/978-1-62703-574-3_1
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-573-6
Online ISBN: 978-1-62703-574-3
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