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Culturing of Specialized Glial Cells (Olfactory Ensheathing Cells) of Human Olfactory Epithelium

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

A monolayer of dissociated glial cells of human olfactory epithelium was cultured in Petri dishes and 12-well plates using a polylysine-laminin substrate. Primary cultures were sub-cultured after 10–15 days. The cell cultures were analyzed by phase contrast microscopy at all stages of culturing. A cytological study involved histological methods (trypan blue staining) and immunocytochemical visualization of GFAP, nestin, and low-affinity nerve growth factor receptors. At the final stage of culturing (5 passages) the monolayer cultures included 2 types of cells: GFAP- and p75-positive glial cells and nestin-positive fibroblasts.

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Authors and Affiliations

  1. Department of Biological Psychiatry, V. P. Serbskii State Research Center of Social and Forensic Medicine, Russia

    E. A. Savchenko, T. B. Dmitrieva, I. V. Viktorov &  V.P.Chekhonin

  2. Laboratory of Experimental Neurocytology, Institute of Brain, Russian Academy of Medical Sciences, Moscow, Russia

    N. A. Andreeva & I. V. Viktorov

Authors
  1. E. A. Savchenko
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  2. N. A. Andreeva
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  3. T. B. Dmitrieva
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  4. I. V. Viktorov
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  5. V.P.Chekhonin
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Corresponding author

Correspondence to V.P.Chekhonin.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 95–98, 2005

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Savchenko, E.A., Andreeva, N.A., Dmitrieva, T.B. et al. Culturing of Specialized Glial Cells (Olfactory Ensheathing Cells) of Human Olfactory Epithelium. Bull Exp Biol Med 139, 510–513 (2005). https://doi.org/10.1007/s10517-005-0332-0

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  • DOI: https://doi.org/10.1007/s10517-005-0332-0

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