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Isolation and Culture of Oligodendrocytes

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Oligodendrocytes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1936))

Abstract

Primary cultures of brain-derived rodent cells are widely used to study molecular and cellular mechanisms in neurobiology. In this chapter, we describe methods of purifying and culturing oligodendroglial cells from mouse perinatal brains. In addition, we describe methods of coculturing the purified oligodendrocytes with neurons. When prepared and cultured according to these protocols, many essential aspects of the biology of oligodendrocytes, such as their proliferation, differentiation, and myelination, can be studied in culture.

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Author information

Authors and Affiliations

  1. Max Planck Institute of Experimental Medicine, Goettingen, Germany

    Marie-Theres Weil, Giselheid Schulz-Ëberlin & Mikael Simons

  2. Center Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB), Goettingen, Germany

    Marie-Theres Weil

  3. Institute of Neuronal Cell Biology, Technical University Munich, Munich, Germany

    Chaitali Mukherjee & Mikael Simons

  4. German Center for Neurodegenerative Disease (DZNE), Munich, Germany

    Chaitali Mukherjee & Mikael Simons

  5. Munich Cluster for Systems Neurology (SyNergy), Munich, Germany

    Chaitali Mukherjee & Mikael Simons

  6. Department of Biology, Molecular Cell Biology, Johannes Gutenberg University, Mainz, Germany

    Wen Ping Kuo-Elsner

  7. Institute of Physiology, University Medical Center, Johannes Gutenberg University, Mainz, Germany

    Isabelle Schäfer & Christina Müller

  8. AbbVie Germany GmbH & Co. KG, Knollstrasse, Ludwigshafen, Germany

    Marie-Theres Weil

Authors
  1. Marie-Theres Weil
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  2. Giselheid Schulz-Ëberlin
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  3. Chaitali Mukherjee
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  4. Wen Ping Kuo-Elsner
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  5. Isabelle Schäfer
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  6. Christina Müller
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  7. Mikael Simons
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Corresponding author

Correspondence to Mikael Simons .

Editor information

Editors and Affiliations

  1. Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK

    David A. Lyons

  2. Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK

    Linde Kegel

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Weil, MT. et al. (2019). Isolation and Culture of Oligodendrocytes. In: Lyons, D., Kegel, L. (eds) Oligodendrocytes. Methods in Molecular Biology, vol 1936. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9072-6_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9072-6_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9070-2

  • Online ISBN: 978-1-4939-9072-6

  • eBook Packages: Springer Protocols

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