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Prebiotic, probiotic, and synbiotic in the diet of Nile tilapia post-larvae during the sex reversal phase

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Abstract

This study evaluated the use of the prebiotic Active-MOS® (mannanoligosaccharides—Biorigin®) and two probiotics: PAS-TR® (Bacillus subtilis and Bacillus cereus var. toyoi—Imeve®) and Bioplus 2BC® (1.6 × 1010 UFC g−1 de Bacillus subtilis and 1.6 × 1010 UFC g−1 Bacillus licheniformis—Christian Hansen®) tested separately and together, in the diet of Nile tilapia post-larvae during the sex reversal phase. The experiment was conducted in two stages: (i) a total of 2160 3-day-old post-larvae (PL) (10.39 ± 0.85 mm and 12.28 ± 3.15 mg) were used and distributed in 24 tanks of 40 L each (3.0 PL L−1). Growth performance, chemical analysis of carcass, bacterial recovery, and histomorphometry of intestinal villi were evaluated; (ii) 240 tilapia (4.28 ± 0.19 cm and 1.19 ± 0.09 g) from the previous experiment were used and stocked at 10 PL per aquarium. The parameters evaluated were survival and relative protection level after bacterial challenge against Aeromonas hydrophila. Six treatments with four replications in a completely randomized design were used for both experimental stages. Additives in the diet of tilapia post-larvae did not determine significant differences in growth, survival, microbiological, or histomorphometric parameters in this study. Nevertheless, after the experimental infection, advantages on the use of the additives were observed in terms of higher relative protection levels (38.10%) and relative percent survival in fish receiving Active-MOS® + Bioplus 2BC®. Therefore, we recommend the use of synbiotic (Active-MOS® + Bioplus 2BC®) in the farming of Nile tilapia PL with recurrent outbreaks of bacterial diseases during the sex reversal phase.

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Authors and Affiliations

  1. Instituto de Pesca, Agência Paulista de Tecnologia dos Agronegócios, Secretaria de Agricultura e Abastecimento, Av. Francisco Matarazzo, 455, São Paulo, SP, 05001-900, Brazil

    Ednara Ronise Lima de Araújo, Carlos Massatoshi Ishikawa, Danielle de Carla Dias, Fábio Rosa Sussel, Hélcio Luis de Almeida Marques & Leonardo Tachibana

  2. Laboratório de Aquacultura de Espécies Tropicais, Instituto Federal de Educação Ciência e Tecnologia do Pará, IFPA Campus Castanhal, BR 316, Km 61 S/N, Castanhal, PA, 68740-970, Brazil

    Luis André Luz Barbas

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  1. Ednara Ronise Lima de Araújo
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  2. Luis André Luz Barbas
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  3. Carlos Massatoshi Ishikawa
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  4. Danielle de Carla Dias
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Correspondence to Ednara Ronise Lima de Araújo.

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The experiments were approved by the Ethical and Animal Welfare Committee of the Instituto de Pesca de São Paulo (# 01/2015).

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de Araújo, E.R.L., Barbas, L.A.L., Ishikawa, C.M. et al. Prebiotic, probiotic, and synbiotic in the diet of Nile tilapia post-larvae during the sex reversal phase. Aquacult Int 26, 85–97 (2018). https://doi.org/10.1007/s10499-017-0201-7

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