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Influence of the dietary inclusion of Gracilaria cornea and Ulva rigida on the biodiversity of the intestinal microbiota of Sparus aurata juveniles

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Abstract

Partial substitutions of fish meal by 5, 15, or 25 % of Gracilaria cornea or Ulva rigida in experimental diets were evaluated to study their effects on biodiversity of intestinal microbiota composition in gilthead seabream (Sparus aurata) juveniles. The diets were offered to duplicate groups of 15 juvenile fish (14.0 ± 0.5 g) for 70 days, and at the end of the experiment the intestinal microbiota from four specimens of each treatment was analysed by denaturing gel gradient electrophoresis. Results showed that the substitution of fish meal by algae meal induced important modifications in the intestinal microbiota community, as a big reduction of the biodiversity when the highest percentage (25 %) of U. rigida was included. On the contrary, an increase on the number of species was detected when a 15 % of algae was included. Various Lactobacillus delbrueckii subspecies were selectively stimulated when G. cornea was included in the feed, and other bacterial species, such as those included in the Vibrio genus, were reduced.

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Abbreviations

DGGE:

Denaturing gel gradient electrophoresis

Fc:

Functional organization

FCR:

Food conversion rate

NCBI:

National Center for Biotechnology Information

PBS:

Phosphate buffered saline

PCR:

Polymerase chain reaction

PER:

Protein efficiency rate

R :

Specific richness

Rr:

Range-weighted richness

SGR:

Specific growth rate

UPGMA:

Unweighted pair groups method arithmetic mean

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Authors and Affiliations

  1. Department of Ecology and Geology, University of Málaga, 29071, Málaga, Spain

    R. M. Rico, F. López-Figueroa & R. T. Abdala-Díaz

  2. Department of Microbiology, Faculty of Sciences, University of Málaga, Campus de Teatinos, 29071, Málaga, Spain

    S. T. Tapia-Paniagua, M. C. Balebona & M. A. Moriñigo

  3. Department of Microbiology, Faculty of Veterinary, University of Las Palmas de Gran Canaria, 35080 Las Palmas de Gran Canaria, Canary Islands, Spain

    M. T. Tejedor-Junco

  4. Department of Applied Biology, University of Almería, 04120, Almería, Spain

    F. J. Alarcón

  5. Department of Biology, Faculty of Marine and Environmental Sciences, University of Cádiz, Campus de Excelencia Internacional del Mar (CEIMAR), 11510, Cádiz, Spain

    J. M. Mancera

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  1. R. M. Rico
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Rico, R.M., Tejedor-Junco, M.T., Tapia-Paniagua, S.T. et al. Influence of the dietary inclusion of Gracilaria cornea and Ulva rigida on the biodiversity of the intestinal microbiota of Sparus aurata juveniles. Aquacult Int 24, 965–984 (2016). https://doi.org/10.1007/s10499-015-9964-x

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