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Long non-coding RNA SNHG4 enhances RNF14 mRNA stability to promote the progression of colorectal cancer by recruiting TAF15 protein

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Abstract

SNHG4 is a lncRNA that was previously reported to promote colorectal cancer (CRC) progression via molecular sponge mechanism. Bioinformatic analysis suggested SNHG4 might scaffold TAF15 protein-RNF14 mRNA interaction. We aimed to investigate the mechanisms of potential SNHG4/TAF15/RNF14 axis in promoting CRC malignant phenotypes. Protein-RNA interaction was determined using RNA immunoprecipitation, pull-down and fluorescence in situ hybridization (FISH) combined immunofluorescence assays. Cell apoptosis rates were quantified using flow cytometry. CCK-8 and colony formation were adopted to determine cell proliferation. Wound healing and transwell assays were employed to assess cell migration and invasion, respectively. Xenograft tumor model was applied to assess the effects of SNHG4 on CRC tumorigenesis in vivo. SNHG4, TAF15 and RNF14 were up-regulated in CRC tissues. SNHG4 overexpression promoted cell proliferation, migration, invasion, and Wnt/β-catenin pathway activation in vitro, as well as tumor growth in vivo. The inhibited malignant phenotypes caused by SNHG4 knockdown were impeded by TAF15 or RNF14 overexpression. Mechanistically, SNHG4 recruited TAF15 protein and thus promoted the interaction between TAF15 protein and RNF14 mRNA, leading to the increased RNF14 mRNA stability. This in turn facilitated the Wnt/β-catenin signal transduction. SNHG4 enhanced RNF14 mRNA stability and activated the Wnt/β-catenin pathway to promote the progression of colorectal cancer by recruiting TAF15 protein.

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Data availability

All data generated or analyzed during this study are included in this article. The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

CRC:

Colorectal cancer

FISH:

Fluorescence in situ hybridization

lncRNAs:

Long non-coding RNAs

EMT:

Epithelial-mesenchymal transition

SNHG4:

Small nucleolar RNA host gene 4

TAF15:

TATA-box binding protein associated factor 15

TCF/LEF:

T-cell factor/lymphoid enhancer-binding factor

RNF:

Ring finger

DMEM:

Dulbecco’s Modified Eagle’s Medium

RL:

Right lower

RU:

Right upper

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Acknowledgements

Not applicable.

Funding

This work was supported by Guangxi Research Foundation for Science &Technology Base and Talent Special (no. AD20238005).

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Authors and Affiliations

  1. Department of Breast and Thyroid Surgery, Liuzhou People’s Hospital, NO.8, Wenchang Road, Liuzhou, 545006, Guangxi, People’s Republic of China

    Lv Lv & Li Zhang

  2. Department of Gastrointestinal Surgery, Affiliated Hospital of Guilin Medical University, Guilin, 541001, Guangxi, People’s Republic of China

    Bojie Huang

  3. Department of Dermatology & Venerology, Affiliated Hospital of Guilin Medical University, Guilin, 541001, Guangxi, People’s Republic of China

    Lu Yi

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Contributions

LL: Conceptualization; Writing-original draft; Methodology; Formal analysis; Supervision; Validation; Visualization; BH: Data curation; Resources; LY: Investigation; Software; LZ: Funding acquisition; Project administration; Writing-review & editing.

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Correspondence to Lv Lv.

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The authors declare that they have no conflict of interest.

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The approval for patient recruitment, specimen collection and tissue processing has been acquired from the clinical research ethics committee in Affiliated Hospital of Guilin Medical University. The animal research ethics committee of Affiliated Hospital of Guilin Medical University approved all the animal experiment procedures.

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Lv, L., Huang, B., Yi, L. et al. Long non-coding RNA SNHG4 enhances RNF14 mRNA stability to promote the progression of colorectal cancer by recruiting TAF15 protein. Apoptosis 28, 414–431 (2023). https://doi.org/10.1007/s10495-022-01781-6

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